Mesoporous Silica Promotes Osteogenesis of Human Adipose-Derived Stem Cells Identified by a High-Throughput Microfluidic Chip Assay

Overview

Journal Pharmaceutics

Publisher MDPI

Date 2022 Dec 23

PMID 36559224

Authors

Xin Chen

Chao Wang

Min Hao

Hang Zhao

He Xia

Liyang Yu

Dong Li

Jichuan Qiu

Haijun Li

Lin Han

Yuanhua Sang

Affiliations

Soon will be listed here.

Abstract

Silicon-derived biomaterials are conducive to regulating the fate of osteo-related stem cells, while their effects on the osteogenic differentiation of human adipose-derived stem cells (hADSCs) remain inconclusive. Mesoporous silica (mSiO) is synthesized in a facile route that exhibited the capability of promoting osteogenic differentiation of hADSCs. The metabolism of SiO in cells is proposed according to the colocalization fluorescence analysis between lysosomes and nanoparticles. The released silicon elements promote osteogenic differentiation. The detection of secretory proteins through numerous parallel experiments performed via a microfluidic chip confirms the positive effect of SiO on the osteogenic differentiation of hADSCs. Moreover, constructed with superparamagnetic iron oxide (FeO), the magnetic nanoparticles (MNPs) of FeO@mSiO endow the cells with magnetic resonance imaging (MRI) properties. The MNP-regulated osteogenic differentiation of autologous adipose-derived stem cells provides considerable clinical application prospects for stem cell therapy of bone tissue repair with an effective reduction in immune rejection.

Citing Articles

Cytocompatible and osteoconductive silicon oxycarbide glass scaffolds 3D printed by DLP: a potential material for bone tissue regeneration.

Carnieri M, Garcia D, Voltolini R, Volpato N, Mafra M, Bernardelli E Front Bioeng Biotechnol. 2024; 11:1297327.

PMID: 38239914 PMC: 10794595. DOI: 10.3389/fbioe.2023.1297327.

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