Investigation of Methods to Extract Confocal Function Parameters for the Depth Resolved Determination of Attenuation Coefficients Using OCT in Intralipid Samples, Titanium Oxide Phantoms, and in Vivo Human Retinas

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2021 Dec 3

PMID 34858682

Citations 7

Authors

Johannes Kubler

Vincent S Zoutenbier

Arjen Amelink

Jorg Fischer

Johannes F de Boer

Affiliations

Soon will be listed here.

Abstract

The attenuation coefficient provides a quantitative parameter for tissue characterization and can be calculated from optical coherence tomography (OCT) data, but accurate determination requires compensation for the confocal function. We present extensive measurement series for extraction of the focal plane and the apparent Rayleigh length from the ratios of OCT images acquired with different focus depths and compare these results with two alternative approaches. By acquiring OCT images for a range of different focus depths the optimal focus plane difference is determined for intralipid and titanium oxide (TiO) phantoms with different scatterer concentrations, which allows for calculation of the attenuation coefficient corrected for the confocal function. The attenuation coefficient is determined for homogeneous intralipid and TiO samples over a wide range of concentrations. We further demonstrate very good reproducibility of the determined attenuation coefficient of layers with identical scatter concentrations in a multi-layered phantom. Finally, this method is applied to in vivo retinal data.

Citing Articles

Basis function model to extract the combined confocal and fall-off function from multiple optical coherence tomography A-scans.

Phan D, Were M, Weitkamp J, Bowden A J Biomed Opt. 2025; 30(2):025003.

PMID: 40027922 PMC: 11868661. DOI: 10.1117/1.JBO.30.2.025003.

Chromatic dispersion based axial length estimation using retinal spectral domain optical coherence tomography.

Kubler J, Fischer J, de Boer J Biomed Opt Express. 2025; 16(2):793-805.

PMID: 39958855 PMC: 11828442. DOI: 10.1364/BOE.553735.

Optical coherence tomography enables longitudinal evaluation of cell graft-directed remodeling in stroke lesions.

Adewumi H, Simkulet M, Kureli G, Giblin J, Lopez A, Erdener S Exp Neurol. 2024; 385:115117.

PMID: 39694221 PMC: 11781960. DOI: 10.1016/j.expneurol.2024.115117.

Accurate attenuation characterization in optical coherence tomography using multi-reference phantoms and deep learning.

Peng N, Xu C, Shen Y, Yuan W, Yang X, Qi C Biomed Opt Express. 2024; 15(12):6697-6714.

PMID: 39679392 PMC: 11640581. DOI: 10.1364/BOE.543606.

Theoretical and experimental determination of the confocal function of OCT systems for accurate calculation of sample optical properties.

Buist G, Debiasi M, Amelink A, de Boer J Biomed Opt Express. 2024; 15(5):2937-2957.

PMID: 38855667 PMC: 11161342. DOI: 10.1364/BOE.516229.

References

Girard M, Strouthidis N, Ethier C, Mari J . Shadow removal and contrast enhancement in optical coherence tomography images of the human optic nerve head. Invest Ophthalmol Vis Sci. 2011; 52(10):7738-48. DOI: 10.1167/iovs.10-6925. View

Wojtkowski M, Leitgeb R, Kowalczyk A, Bajraszewski T, Fercher A . In vivo human retinal imaging by Fourier domain optical coherence tomography. J Biomed Opt. 2002; 7(3):457-63. DOI: 10.1117/1.1482379. View

Almasian M, Bosschaart N, van Leeuwen T, Faber D . Validation of quantitative attenuation and backscattering coefficient measurements by optical coherence tomography in the concentration-dependent and multiple scattering regime. J Biomed Opt. 2016; 20(12):121314. DOI: 10.1117/1.JBO.20.12.121314. View

de Boer J, Saxer C, Nelson J . Stable carrier generation and phase-resolved digital data processing in optical coherence tomography. Appl Opt. 2008; 40(31):5787-90. DOI: 10.1364/ao.40.005787. View

Stefan S, Jeong K, Polucha C, Tapinos N, Toms S, Lee J . Determination of confocal profile and curved focal plane for OCT mapping of the attenuation coefficient. Biomed Opt Express. 2018; 9(10):5084-5099. PMC: 6179411. DOI: 10.1364/BOE.9.005084. View

van der Schoot J, Vermeer K, de Boer J, Lemij H . The effect of glaucoma on the optical attenuation coefficient of the retinal nerve fiber layer in spectral domain optical coherence tomography images. Invest Ophthalmol Vis Sci. 2012; 53(4):2424-30. DOI: 10.1167/iovs.11-8436. View

Sun M, Zhang Z, Ma C, Chen S, Chen X . Quantitative analysis of retinal layers on three-dimensional spectral-domain optical coherence tomography for pituitary adenoma. PLoS One. 2017; 12(6):e0179532. PMC: 5476276. DOI: 10.1371/journal.pone.0179532. View

Yun S, Tearney G, Bouma B, Park B, de Boer J . High-speed spectral-domain optical coherence tomography at 1.3 mum wavelength. Opt Express. 2009; 11(26):3598-604. PMC: 2713046. DOI: 10.1364/oe.11.003598. View

Ghafaryasl B, Vermeer K, Kalkman J, Callewaert T, de Boer J, van Vliet L . Analysis of attenuation coefficient estimation in Fourier-domain OCT of semi-infinite media. Biomed Opt Express. 2020; 11(11):6093-6107. PMC: 7687928. DOI: 10.1364/BOE.403283. View

10.

Vermeer K, Mo J, Weda J, Lemij H, de Boer J . Depth-resolved model-based reconstruction of attenuation coefficients in optical coherence tomography. Biomed Opt Express. 2014; 5(1):322-37. PMC: 3891343. DOI: 10.1364/BOE.5.000322. View

11.

Nassif N, Cense B, Park B, Yun S, Chen T, Bouma B . In vivo human retinal imaging by ultrahigh-speed spectral domain optical coherence tomography. Opt Lett. 2004; 29(5):480-2. DOI: 10.1364/ol.29.000480. View

12.

de Bruin D, Bremmer R, Kodach V, de Kinkelder R, van Marle J, van Leeuwen T . Optical phantoms of varying geometry based on thin building blocks with controlled optical properties. J Biomed Opt. 2010; 15(2):025001. DOI: 10.1117/1.3369003. View

13.

Faber D, van der Meer F, Aalders M, van Leeuwen T . Quantitative measurement of attenuation coefficients of weakly scattering media using optical coherence tomography. Opt Express. 2009; 12(19):4353-65. DOI: 10.1364/opex.12.004353. View

14.

Chiu S, Li X, Nicholas P, Toth C, Izatt J, Farsiu S . Automatic segmentation of seven retinal layers in SDOCT images congruent with expert manual segmentation. Opt Express. 2010; 18(18):19413-28. PMC: 3408910. DOI: 10.1364/OE.18.019413. View

15.

Vermeer K, van der Schoot J, Lemij H, de Boer J . RPE-normalized RNFL attenuation coefficient maps derived from volumetric OCT imaging for glaucoma assessment. Invest Ophthalmol Vis Sci. 2012; 53(10):6102-8. DOI: 10.1167/iovs.12-9933. View

16.

Smith G, Dwork N, OConnor D, Sikora U, Lurie K, Pauly J . Automated, Depth-Resolved Estimation of the Attenuation Coefficient From Optical Coherence Tomography Data. IEEE Trans Med Imaging. 2015; 34(12):2592-602. PMC: 4714956. DOI: 10.1109/TMI.2015.2450197. View

17.

Hillmann D, Bonin T, Luhrs C, Franke G, Hagen-Eggert M, Koch P . Common approach for compensation of axial motion artifacts in swept-source OCT and dispersion in Fourier-domain OCT. Opt Express. 2012; 20(6):6761-76. DOI: 10.1364/OE.20.006761. View

18.

Tomlins P, Adegun O, Hagi-Pavli E, Piper K, Bader D, Fortune F . Scattering attenuation microscopy of oral epithelial dysplasia. J Biomed Opt. 2011; 15(6):066003. DOI: 10.1117/1.3505019. View

19.

Dwork N, Smith G, Leng T, Pauly J, Bowden A . Automatically Determining the Confocal Parameters From OCT B-Scans for Quantification of the Attenuation Coefficients. IEEE Trans Med Imaging. 2018; 38(1):261-268. PMC: 11465109. DOI: 10.1109/TMI.2018.2861570. View

20.

Nguyen V, Faber D, van der Pol E, van Leeuwen T, Kalkman J . Dependent and multiple scattering in transmission and backscattering optical coherence tomography. Opt Express. 2014; 21(24):29145-56. DOI: 10.1364/OE.21.029145. View