Genome-wide Nuclear Data Confirm Two Species in the Alpine Endemic Land Snail S.l. (Gastropoda, Hygromiidae)

Overview

Journal J Zool Syst Evol Res

Date 2021 Dec 2

PMID 34853493

Citations 4

Authors

Sonja Bamberger

Michael Duda

Andreas Tribsch

Elisabeth Haring

Helmut Sattmann

Oliver Macek

Matthias Affenzeller

Luise Kruckenhauser

Affiliations

Soon will be listed here.

Abstract

The Austrian endemic land snail species (formerly ) occurs in the Northeastern Calcareous Alps at high elevations. Two morphologically highly similar subspecies and have been described. First analyses of mitochondrial and nuclear marker sequences indicated a high genetic divergence between them. In the present study, we aimed to assess gene flow between the two subspecies which should help to re-evaluate their taxonomic status. Sequence data and amplified fragment length polymorphism (AFLP) markers of 255 specimens covering the whole distribution range were analyzed. A clear geographic separation was found within the potential contact zone, the Haller Mauern mountain range. Samples of all western sites were part of the clade representing and almost all samples from the eastern sites clustered with . However, within two sampling sites of the eastern Haller Mauern, a few individuals possessed a sequence matching the clade whereas at the locus they were heterozygous possessing the alleles of both taxa. Contrary to the results indicating historical and/or ongoing hybridization, AFLP analyses of 202 individuals confirmed a clear separation of the two taxa congruent with the mitochondrial data. Although they occur on the same mountain range without any physical barrier, no indication of ongoing gene flow between the two taxa was found. Thus, we conclude that the two taxa are separate species and .

Citing Articles

On the verge of extinction - revision of a highly endangered Swiss alpine snail with description of a new genus, gen. nov. (Gastropoda, Eupulmonata, Hygromiidae).

Kneubuhler J, Baggenstos M, Neubert E Zookeys. 2023; 1104:69-91.

PMID: 36761925 PMC: 9848827. DOI: 10.3897/zookeys.1104.82866.

Genome-wide nuclear data confirm two species in the Alpine endemic land snail s.l. (Gastropoda, Hygromiidae).

Bamberger S, Duda M, Tribsch A, Haring E, Sattmann H, Macek O J Zool Syst Evol Res. 2021; 58(4):982-1004.

PMID: 34853493 PMC: 8609433. DOI: 10.1111/jzs.12362.

Deep genetic structure at a small spatial scale in the endangered land snail Xerocrassa montserratensis.

Catala C, Bros V, Castelltort X, Santos X, Pascual M Sci Rep. 2021; 11(1):8855.

PMID: 33893328 PMC: 8065133. DOI: 10.1038/s41598-021-87741-7.

Redescription of (De Stefani, 1879), a species endemic to the central Apennines, Italy (Gastropoda, Eupulmonata, Hygromiidae) by an integrative molecular and morphological approach.

Pienkowska J, Manganelli G, Giusti F, Barbato D, Kosicka E, Hallgass A Zookeys. 2020; 988:17-61.

PMID: 33223890 PMC: 7666099. DOI: 10.3897/zookeys.988.56397.

References

Evanno G, Regnaut S, Goudet J . Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol. 2005; 14(8):2611-20. DOI: 10.1111/j.1365-294X.2005.02553.x. View

Neiber M, Razkin O, Hausdorf B . Molecular phylogeny and biogeography of the land snail family Hygromiidae (Gastropoda: Helicoidea). Mol Phylogenet Evol. 2017; 111:169-184. DOI: 10.1016/j.ympev.2017.04.002. View

Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S . Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics. 2012; 28(12):1647-9. PMC: 3371832. DOI: 10.1093/bioinformatics/bts199. View

Yao H, Song J, Liu C, Luo K, Han J, Li Y . Use of ITS2 region as the universal DNA barcode for plants and animals. PLoS One. 2010; 5(10). PMC: 2948509. DOI: 10.1371/journal.pone.0013102. View

Kumar S, Stecher G, Tamura K . MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol. 2016; 33(7):1870-4. PMC: 8210823. DOI: 10.1093/molbev/msw054. View

Bendiksby M, Tribsch A, Borgen L, Travnicek P, Brysting A . Allopolyploid origins of the Galeopsis tetraploids--revisiting Müntzing's classical textbook example using molecular tools. New Phytol. 2011; 191(4):1150-1167. DOI: 10.1111/j.1469-8137.2011.03753.x. View

Bamberger S, Duda M, Tribsch A, Haring E, Sattmann H, Macek O . Genome-wide nuclear data confirm two species in the Alpine endemic land snail s.l. (Gastropoda, Hygromiidae). J Zool Syst Evol Res. 2021; 58(4):982-1004. PMC: 8609433. DOI: 10.1111/jzs.12362. View

Rozas J, Rozas R . DnaSP, DNA sequence polymorphism: an interactive program for estimating population genetics parameters from DNA sequence data. Comput Appl Biosci. 1995; 11(6):621-5. DOI: 10.1093/bioinformatics/11.6.621. View

Kruckenhauser L, Duda M, Bartel D, Sattmann H, Harl J, Kirchner S . Paraphyly and budding speciation in the hairy snail (Pulmonata, Hygromiidae). Zool Scr. 2014; 43(3):273-288. PMC: 4144147. DOI: 10.1111/zsc.12046. View

10.

Peakall R, Smouse P . GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research--an update. Bioinformatics. 2012; 28(19):2537-9. PMC: 3463245. DOI: 10.1093/bioinformatics/bts460. View

11.

Pompanon F, Bonin A, Bellemain E, Taberlet P . Genotyping errors: causes, consequences and solutions. Nat Rev Genet. 2005; 6(11):847-59. DOI: 10.1038/nrg1707. View

12.

Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M . AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 1995; 23(21):4407-14. PMC: 307397. DOI: 10.1093/nar/23.21.4407. View

13.

Duda M, Kruckenhauser L, Haring E, Sattmann H . Habitat requirements of the pulmonate land snails and endemic to the Northern Calcareous Alps, Austria. Eco Mont. 2015; 2(2):5-12. PMC: 4340512. DOI: 10.1553/eco.mont-2-2s5. View

14.

Bandelt H, Forster P, Rohl A . Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol. 1999; 16(1):37-48. DOI: 10.1093/oxfordjournals.molbev.a026036. View

15.

Gittenberger E, Piel W, Groenenberg D . The Pleistocene glaciations and the evolutionary history of the polytypic snail species Arianta arbustorum (Gastropoda, Pulmonata, Helicidae). Mol Phylogenet Evol. 2004; 30(1):64-73. DOI: 10.1016/s1055-7903(03)00182-9. View

16.

Librado P, Rozas J . DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 2009; 25(11):1451-2. DOI: 10.1093/bioinformatics/btp187. View

17.

Long E, Dawid I . Repeated genes in eukaryotes. Annu Rev Biochem. 1980; 49:727-64. DOI: 10.1146/annurev.bi.49.070180.003455. View

18.

Coleman A, Vacquier V . Exploring the phylogenetic utility of ITS sequences for animals: a test case for abalone (Haliotis). J Mol Evol. 2002; 54(2):246-57. DOI: 10.1007/s00239-001-0006-0. View

19.

Meirmans P . Seven common mistakes in population genetics and how to avoid them. Mol Ecol. 2015; 24(13):3223-31. DOI: 10.1111/mec.13243. View

20.

Hausdorf B . Progress toward a general species concept. Evolution. 2011; 65(4):923-31. DOI: 10.1111/j.1558-5646.2011.01231.x. View