High-resolution Structures of the Actomyosin-V Complex in Three Nucleotide States Provide Insights into the Force Generation Mechanism

Overview

Journal Elife

Specialty Biology

Date 2021 Nov 23

PMID 34812732

Citations 20

Authors

Sabrina Pospich

H Lee Sweeney

Anne Houdusse

Stefan Raunser

Affiliations

Soon will be listed here.

Abstract

The molecular motor myosin undergoes a series of major structural transitions during its force-producing motor cycle. The underlying mechanism and its coupling to ATP hydrolysis and actin binding are only partially understood, mostly due to sparse structural data on actin-bound states of myosin. Here, we report 26 high-resolution cryo-EM structures of the actomyosin-V complex in the strong-ADP, rigor, and a previously unseen post-rigor transition state that binds the ATP analog AppNHp. The structures reveal a high flexibility of myosin in each state and provide valuable insights into the structural transitions of myosin-V upon ADP release and binding of AppNHp, as well as the actomyosin interface. In addition, they show how myosin is able to specifically alter the structure of F-actin.

Citing Articles

High-resolution structures of Myosin-IC reveal a unique actin-binding orientation, ADP release pathway, and power stroke trajectory.

Chavali S, Carman P, Shuman H, Ostap E, Sindelar C Proc Natl Acad Sci U S A. 2025; 122(9):e2415457122.

PMID: 40014570 PMC: 11892617. DOI: 10.1073/pnas.2415457122.

High resolution structures of Myosin-IC reveal a unique actin-binding orientation, ADP release pathway, and power stroke trajectory.

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