Isolation and Characterization of CDNA Clones for Human Erythrocyte Beta-spectrin

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1987 Nov 1

PMID 3478706

Citations 10

Authors

J T Prchal

B J Morley

S H Yoon

T L Coetzer

J PALEK

J G Conboy

Y W Kan

Affiliations

Soon will be listed here.

Abstract

Spectrin is an important structural component of the membrane skeleton that underlies and supports the erythrocyte plasma membrane. It is composed of nonidentical alpha (Mr 240,000) and beta (Mr 220,000) subunits, each of which contains multiple homologous 106-amino acid segments. We report here the isolation and characterization of a human erythroid-specific beta-spectrin cDNA clone that encodes parts of the beta-9 through beta-12 repeat segments. This cDNA was used as a hybridization probe to assign the beta-spectrin gene to human chromosome 14 and to begin molecular analysis of the gene and its mRNA transcripts. RNA transfer blot analysis showed that the reticulocyte beta-spectrin mRNA is 7.8 kilobases in length. Southern blot analysis of genomic DNA revealed the presence of restriction fragment length polymorphisms (RFLPs) within the beta-spectrin gene locus. The isolation of human spectrin cDNA probes and the identification of closely linked RFLPs will facilitate analysis of mutant spectrin genes causing congenital hemolytic anemias associated with quantitative and qualitative spectrin abnormalities.

Citing Articles

A novel isoform of beta-spectrin II localizes to cerebellar Purkinje-cell bodies and interacts with neurofibromatosis type 2 gene product schwannomin.

Chen Y, Yu P, Lu D, Tagle D, Cai T J Mol Neurosci. 2001; 17(1):59-70.

PMID: 11665863 DOI: 10.1385/JMN:17:1:59.

A TaqI polymorphism in the human erythroid beta spectrin gene.

Beeton L, Prchal J, Coetzer T Hum Genet. 1995; 95(3):365-6.

PMID: 7868137 DOI: 10.1007/BF00225212.

Detection and expression of a cDNA clone that encodes a polypeptide containing two inhibitory domains of human calpastatin and its recognition by rheumatoid arthritis sera.

Despres N, Talbot G, Plouffe B, Boire G, Menard H J Clin Invest. 1995; 95(4):1891-6.

PMID: 7706496 PMC: 295733. DOI: 10.1172/JCI117870.

Chromosomal location of three spectrin genes: relationship to the inherited hemolytic anemias of mouse and man.

Birkenmeier C, Barker J Proc Natl Acad Sci U S A. 1988; 85(21):8121-5.

PMID: 3186715 PMC: 282367. DOI: 10.1073/pnas.85.21.8121.

Unequal synthesis and differential degradation of alpha and beta spectrin during murine erythroid differentiation.

Lehnert M, Lodish H J Cell Biol. 1988; 107(2):413-26.

PMID: 3166462 PMC: 2115221. DOI: 10.1083/jcb.107.2.413.

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