Authentic Beta-globin MRNA Sequences in Homozygous BetaO-thalassemia

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1977 Jul 1

PMID 268654

Citations 26

Authors

G F Temple

J C Chang

Y W Kan

Affiliations

Soon will be listed here.

Abstract

In a patient with homozygous betaO-thalassemia in whom studies of reticulocyte hemoglobin synthesis showed no beta-globin chain synthesis in vivo and in vitro, molecular hybridization studies revealed RNA sequences complementary to beta-globin cDNA. The fact that these sequences were authentic beta-globin mRNA was shown by fingerprint analysis of T1 ribonuclease-digested mRNA and by sequencing of oligonucleotides unique to beta-globin mRNA. The beta-mRNA that failed to direct beta-globin chain synthesis was not detectably shortened or degraded and contained poly(A) sequences.

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