High-resolution Epitope Mapping by AllerScan Reveals Relationships Between IgE and IgG Repertoires During Peanut Oral Immunotherapy

Overview

Journal Cell Rep Med

Publisher Cell Press

Specialties Biology
General Medicine

Date 2021 Nov 10

PMID 34755130

Citations 23

Authors

Genghao Chen

Ellen L Shrock

Mamie Z Li

Jonathan M Spergel

Kari C Nadeau

Jacqueline A Pongracic

Dale T Umetsu

Rima Rachid

Andrew J MacGinnitie

Wanda Phipatanakul

Lynda Schneider

Hans C Oettgen

Stephen J Elledge

Affiliations

Soon will be listed here.

Abstract

Peanut allergy can result in life-threatening reactions and is a major public health concern. Oral immunotherapy (OIT) induces desensitization to food allergens through administration of increasing amounts of allergen. To dissect peanut-specific immunoglobulin E (IgE) and IgG responses in subjects undergoing OIT, we have developed AllerScan, a method that leverages phage-display and next-generation sequencing to identify the epitope targets of peanut-specific antibodies. We observe a striking diversification and boosting of the peanut-specific IgG repertoire after OIT and a reduction in pre-existing IgE levels against individual epitopes. High-resolution epitope mapping reveals shared recognition of public epitopes in Ara h 1, 2, 3, and 7. In individual subjects, OIT-induced IgG specificities overlap extensively with IgE and exhibit strikingly similar antibody footprints, suggesting related clonal lineages or convergent evolution of peanut-specific IgE and IgG B cells. Individual differences in epitope recognition identified via AllerScan could inform safer and more effective personalized immunotherapy.

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