Interaction Between Two Different Regulatory Elements Activates the Murine Alpha A-crystallin Gene Promoter in Explanted Lens Epithelia

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1987 May 1

PMID 3474517

Citations 15

Authors

A B Chepelinsky

B Sommer

J Piatigorsky

Affiliations

Soon will be listed here.

Abstract

Previous experiments have indicated that 5' flanking DNA sequences (nucleotides-366 to +46) are capable of regulating the lens-specific transcription of the murine alpha A-crystallin gene. Here we have analyzed these 5' regulatory sequences by transfecting explanted embryonic chicken lens epithelia with different alpha A-crystallin-CAT (chloramphenicol acetyltransferase) hybrid genes (alpha A-crystallin promoter sequences fused to the bacterial CAT gene in the pSVO-CAT expression vector). The results indicated the presence of a proximal (-88 to +46) and a distal (-111 to -88) domain which must interact for promoter function. Deletion experiments showed that the sequence between -88 and -60 was essential for function of the proximal domain in the explanted epithelia. A synthetic oligonucleotide containing the sequence between -111 and -84 activated the proximal domain when placed in either orientation 57 base pairs upstream from position -88 of the alpha A-crystallin-CAT hybrid gene.

Citing Articles

Hydrogen peroxide-induced expression of the proto-oncogenes, c-jun, c-fos and c-myc in rabbit lens epithelial cells.

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Functional redundancy of the DE-1 and alpha A-CRYBP1 regulatory sites of the mouse alpha A-crystallin promoter.

Sax C, Ilagan J, Piatigorsky J Nucleic Acids Res. 1993; 21(11):2633-40.

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A complex array of positive and negative elements regulates the chicken alpha A-crystallin gene: involvement of Pax-6, USF, CREB and/or CREM, and AP-1 proteins.

Cvekl A, Sax C, Bresnick E, Piatigorsky J Mol Cell Biol. 1994; 14(11):7363-76.

PMID: 7935450 PMC: 359271. DOI: 10.1128/mcb.14.11.7363-7376.1994.

Lens-specific activity of the mouse alpha A-crystallin promoter in the absence of a TATA box: functional and protein binding analysis of the mouse alpha A-crystallin PE1 region.

Sax C, Cvekl A, Kantorow M, Ilagan J, Ambulos Jr N, Piatigorsky J Nucleic Acids Res. 1995; 23(3):442-51.

PMID: 7885839 PMC: 306695. DOI: 10.1093/nar/23.3.442.

Crystallin genes: lens specificity of the murine alpha A-crystallin gene.

Chepelinsky A, Khillan J, Mahon K, Overbeek P, Westphal H, Piatigorsky J Environ Health Perspect. 1987; 75:17-24.

PMID: 3691437 PMC: 1474452. DOI: 10.1289/ehp.877517.

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