Tissue-specific Activity of the Pro-opiomelanocortin Gene Promoter

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1987 Nov 1

PMID 3431549

Citations 20

Authors

L Jeannotte

M A Trifiro

R K Plante

M Chamberland

J Drouin

Affiliations

Soon will be listed here.

Abstract

The pro-opiomelanocortin (POMC) gene is specifically expressed in corticotroph cells of the anterior pituitary. To define the POMC promoter sequences responsible for tissue-specific expression, we assessed POMC promoter activity by gene transfer into POMC-expressing pituitary tumor cells (AtT-20) and fibroblast L cells. The rat POMC promoter was only efficiently utilized and correctly transcribed in AtT-20 cells. 5'-End deletion analysis revealed two promoter regions required for activity in AtT-20 cells. When tested by fusion to a heterologous promoter, DNA fragments corresponding to both regions exhibited tissue-specific activity, suggesting the presence of at least two tissue-specific DNA sequence elements within the promoter. In summary, POMC promoter sequences from -480 to -34 base pairs appear sufficient to mimic the specificity of anterior pituitary expression.

Citing Articles

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Role of NeuroD1 on the negative regulation of Pomc expression by glucocorticoid.

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Leptin resistance and obesity in mice with deletion of methyl-CpG-binding protein 2 (MeCP2) in hypothalamic pro-opiomelanocortin (POMC) neurons.

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A pituitary-specific enhancer of the POMC gene with preferential activity in corticotrope cells.

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