Tissue- and Species-specific Promoter Elements of Rat Gamma-crystallin Genes

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1990 Mar 11

PMID 2320414

Citations 9

Authors

R Peek

P van der Logt

N H Lubsen

J G Schoenmakers

Affiliations

Soon will be listed here.

Abstract

The 5' flanking regions of the six rat gamma-crystallin genes (gamma A-gamma F) are all capable of conferring lens-specific expression to the bacterial chloramphenicol acetyl transferase (CAT) reporter gene in either transdifferentiating chicken neural retina cells or mouse lens epithelial cells. Deletion mapping of the most active gamma-crystallin promoter region, the gamma D region, showed that at least three elements are required for maximal expression in mouse lens epithelial cells: element(s) located between -200 and -106, a conserved CG rich region around position -75, and a CG stretch around -15. The region between -200 and -106 was dispensable in transdifferentiating chicken neural retina cells, which instead required the region between -106 and -78. The maximal activity of the gamma E and gamma F promoters was also dependent upon the integrity of the conserved CG region located around -75. A synthetic oligonucleotide containing this sequence was capable of lens-specific enhancement of the activity of the tk promoter in transdifferentiating chicken neural retina cells but not in mouse lens epithelial cells. Our results further show that this region may contain a silencer element, active in non-lens tissues, as well.

Citing Articles

Regulation of gene expression by Pax6 in ocular cells: a case of tissue-preferred expression of crystallins in lens.

Cvekl A, Yang Y, Chauhan B, Cveklova K Int J Dev Biol. 2004; 48(8-9):829-44.

PMID: 15558475 PMC: 2080872. DOI: 10.1387/ijdb.041866ac.

c-Maf, the gammaD-crystallin Maf-responsive element and growth factor regulation.

Civil A, van Genesen S, Lubsen N Nucleic Acids Res. 2002; 30(4):975-82.

PMID: 11842109 PMC: 100334. DOI: 10.1093/nar/30.4.975.

Antagonistic action of Six3 and Prox1 at the gamma-crystallin promoter.

Lengler J, Krausz E, Tomarev S, Prescott A, Quinlan R, Graw J Nucleic Acids Res. 2001; 29(2):515-26.

PMID: 11139622 PMC: 29665. DOI: 10.1093/nar/29.2.515.

Analysis of the tumor vasculature and metastatic behavior of xenografts of human melanoma cell lines transfected with vascular permeability factor.

Potgens A, Van Altena M, Lubsen N, Ruiter D, de Waal R Am J Pathol. 1996; 148(4):1203-17.

PMID: 8644861 PMC: 1861532.

Vascular permeability factor expression influences tumor angiogenesis in human melanoma lines xenografted to nude mice.

Potgens A, Lubsen N, Van Altena M, Schoenmakers J, Ruiter D, de Waal R Am J Pathol. 1995; 146(1):197-209.

PMID: 7531947 PMC: 1870766.

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