Plant-derived Human Recombinant Growth Factors and Serum Albumin Maintain Stemness of Human-induced Pluripotent Stem Cells

Overview

Journal Cell Biol Int

Specialty Cell Biology

Date 2021 Oct 25

PMID 34694043

Citations 4

Authors

Yukyeong Lee

Hye Jeong Lee

Seokbeom Ham

Dahee Jeong

Minseong Lee

Uiil Lee

Myungseok Lee

Tae-Ho Kwon

Kinarm Ko

Affiliations

Soon will be listed here.

Abstract

Stem cells are an important therapeutic source for recovery and regeneration, as their ability of self-renewal and differentiation offers an unlimited supply of highly specialized cells for therapeutic transplantation. Growth factors and serum are essential for maintaining the characteristics of stem cells in culture and for inducing differentiation. Because growth factors are produced mainly in bacterial (Escherichia coli) or animal cells, the use of such growth factors raises safety concerns that need to be resolved for the commercialization of stem cell therapeutics. To overcome this problem, studies on proteins produced in plants have been conducted. Here, we describe the functions of plant-derived fibroblast growth factor 2 (FGF2) and human serum albumin in the maintenance and differentiation of human-induced pluripotent stem cells (hiPSCs). Plant-derived FGF2 and human epidermal growth factor EGF were able to differentiate hiPSCs into neural stem cells (NSCs). These NSCs could differentiate into neuronal and glial cells. Our results imply that culturing stem cells in animal-free culture medium, which is composed of plant-derived proteins, would facilitate stem cell application research, for example, for cell therapy, by reducing contamination risk.

Citing Articles

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The Investigation of the Subtle Structural Discrepancies between Oryza Sativa Recombinant and Plasma-Derived Human Serum Albumins to Design a Novel Nanoparticle as a Taxane Delivery System.

Fang R, He L, Wang Y, Wang L, Qian H, Yang S Protein J. 2024; 43(3):544-558.

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Albumin as a Biomaterial and Therapeutic Agent in Regenerative Medicine.

Kuten Pella O, Hornyak I, Horvathy D, Fodor E, Nehrer S, Lacza Z Int J Mol Sci. 2022; 23(18).

PMID: 36142472 PMC: 9502107. DOI: 10.3390/ijms231810557.

Plant-derived human recombinant growth factors and serum albumin maintain stemness of human-induced pluripotent stem cells.

Lee Y, Lee H, Ham S, Jeong D, Lee M, Lee U Cell Biol Int. 2021; 46(1):139-147.

PMID: 34694043 PMC: 9298993. DOI: 10.1002/cbin.11715.

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