Multiplex Protein Analysis for the Study of Glaucoma

Overview

Journal Expert Rev Proteomics

Publisher Informa Healthcare

Date 2021 Oct 21

PMID 34672220

Authors

Gulgun Tezel

Affiliations

Soon will be listed here.

Abstract

Introduction: Glaucoma, a leading cause of irreversible blindness in the world, is a chronic neurodegenerative disease of multifactorial origin. Extensive research is ongoing to better understand, prevent, and treat progressive degeneration of retinal ganglion cells in glaucoma. While experimental models of glaucoma and postmortem tissues of human donors are analyzed for pathophysiological comprehension and improved treatment of this blinding disease, clinical samples of intraocular biofluids and blood collected from glaucoma patients are analyzed to identify predictive, diagnostic, and prognostic biomarkers. Multiplexing techniques for protein analysis offer a valuable approach for translational glaucoma research.

Areas Covered: This review provides an overview of the increasing applications of multiplex protein analysis for glaucoma research and also highlights current research challenges in the field and expected solutions from emerging technological advances.

Expert Opinion: Analytical techniques for multiplex analysis of proteins can help uncover neurodegenerative processes for enhanced treatment of glaucoma and can help identify molecular biomarkers for improved clinical testing and monitoring of this complex disease. This evolving field and continuously growing availability of new technologies are expected to broaden the comprehension of this complex neurodegenerative disease and speed up the progress toward new therapeutics and personalized patient care to prevent blindness from glaucoma.

References

Kompauer M, Heiles S, Spengler B . Atmospheric pressure MALDI mass spectrometry imaging of tissues and cells at 1.4-μm lateral resolution. Nat Methods. 2016; 14(1):90-96. DOI: 10.1038/nmeth.4071. View

Perez-Ruiz E, Decrop D, Ven K, Tripodi L, Leirs K, Rosseels J . Digital ELISA for the quantification of attomolar concentrations of Alzheimer's disease biomarker protein Tau in biological samples. Anal Chim Acta. 2018; 1015:74-81. DOI: 10.1016/j.aca.2018.02.011. View

Futakuchi A, Inoue T, Fujimoto T, Kuroda U, Inoue-Mochita M, Takahashi E . Molecular Mechanisms Underlying the Filtration Bleb-Maintaining Effects of Suberoylanilide Hydroxamic Acid (SAHA). Invest Ophthalmol Vis Sci. 2017; 58(4):2421-2429. DOI: 10.1167/iovs.16-21403. View

Tsang H, Xue V, Koh S, Chiu Y, Ng L, Wong S . NanoString, a novel digital color-coded barcode technology: current and future applications in molecular diagnostics. Expert Rev Mol Diagn. 2016; 17(1):95-103. DOI: 10.1080/14737159.2017.1268533. View

Wang J, Fu M, Liu K, Wang N, Zhang Z, Zhou M . Matricellular Proteins Play a Potential Role in Acute Primary Angle Closure. Curr Eye Res. 2018; 43(6):771-777. DOI: 10.1080/02713683.2018.1449222. View

Yang X, Hondur G, Tezel G . Antioxidant Treatment Limits Neuroinflammation in Experimental Glaucoma. Invest Ophthalmol Vis Sci. 2016; 57(4):2344-54. PMC: 4855827. DOI: 10.1167/iovs.16-19153. View

van Gool A, Corrales F, colovic M, Krstic D, Oliver-Martos B, Martinez-Caceres E . Analytical techniques for multiplex analysis of protein biomarkers. Expert Rev Proteomics. 2020; 17(4):257-273. DOI: 10.1080/14789450.2020.1763174. View

Hondur G, Goktas E, Yang X, Al-Aswad L, Auran J, Blumberg D . Oxidative Stress-Related Molecular Biomarker Candidates for Glaucoma. Invest Ophthalmol Vis Sci. 2017; 58(10):4078-4088. PMC: 5685420. DOI: 10.1167/iovs.17-22242. View

Gramlich O, Beck S, Und Hohenstein-Blaul N, Boehm N, Ziegler A, Vetter J . Enhanced insight into the autoimmune component of glaucoma: IgG autoantibody accumulation and pro-inflammatory conditions in human glaucomatous retina. PLoS One. 2013; 8(2):e57557. PMC: 3581473. DOI: 10.1371/journal.pone.0057557. View

10.

Miedema A, Wijering M, Eggen B, Kooistra S . High-Resolution Transcriptomic and Proteomic Profiling of Heterogeneity of Brain-Derived Microglia in Multiple Sclerosis. Front Mol Neurosci. 2020; 13:583811. PMC: 7654237. DOI: 10.3389/fnmol.2020.583811. View

11.

Micera A, Quaranta L, Esposito G, Floriani I, Pocobelli A, Sacca S . Differential Protein Expression Profiles in Glaucomatous Trabecular Meshwork: An Evaluation Study on a Small Primary Open Angle Glaucoma Population. Adv Ther. 2016; 33(2):252-67. PMC: 4769730. DOI: 10.1007/s12325-016-0285-x. View

12.

Galbis-Estrada C, Pinazo-Duran M, Cantu-Dibildox J, Marco-Ramirez C, Diaz-Llopis M, Benitez-Del-Castillo J . Patients undergoing long-term treatment with antihypertensive eye drops responded positively with respect to their ocular surface disorder to oral supplementation with antioxidants and essential fatty acids. Clin Interv Aging. 2013; 8:711-9. PMC: 3693747. DOI: 10.2147/CIA.S43191. View

13.

Hanrieder J, Phan N, Kurczy M, Ewing A . Imaging mass spectrometry in neuroscience. ACS Chem Neurosci. 2013; 4(5):666-79. PMC: 3656743. DOI: 10.1021/cn400053c. View

14.

Bollinger K, Crabb J, Yuan X, Putliwala T, Clark A, Crabb J . Quantitative proteomics: TGFβ₂ signaling in trabecular meshwork cells. Invest Ophthalmol Vis Sci. 2011; 52(11):8287-94. PMC: 3208113. DOI: 10.1167/iovs.11-8218. View

15.

Baudouin C, Kolko M, Melik-Parsadaniantz S, Messmer E . Inflammation in Glaucoma: From the back to the front of the eye, and beyond. Prog Retin Eye Res. 2020; 83:100916. DOI: 10.1016/j.preteyeres.2020.100916. View

16.

Bollinger K, Crabb J, Yuan X, Putliwala T, Clark A, Crabb J . Proteomic similarities in steroid responsiveness in normal and glaucomatous trabecular meshwork cells. Mol Vis. 2012; 18:2001-11. PMC: 3413418. View

17.

Ma A, McDermaid A, Xu J, Chang Y, Ma Q . Integrative Methods and Practical Challenges for Single-Cell Multi-omics. Trends Biotechnol. 2020; 38(9):1007-1022. PMC: 7442857. DOI: 10.1016/j.tibtech.2020.02.013. View

18.

Inoue T, Kawaji T, Inatani M, Kameda T, Yoshimura N, Tanihara H . Simultaneous increases in multiple proinflammatory cytokines in the aqueous humor in pseudophakic glaucomatous eyes. J Cataract Refract Surg. 2012; 38(8):1389-97. DOI: 10.1016/j.jcrs.2012.04.028. View

19.

Aketa N, Yamaguchi T, Suzuki T, Higa K, Yagi-Yaguchi Y, Satake Y . Iris Damage Is Associated With Elevated Cytokine Levels in Aqueous Humor. Invest Ophthalmol Vis Sci. 2017; 58(6):BIO42-BIO51. DOI: 10.1167/iovs.17-21421. View

20.

Peterson V, Zhang K, Kumar N, Wong J, Li L, Wilson D . Multiplexed quantification of proteins and transcripts in single cells. Nat Biotechnol. 2017; 35(10):936-939. DOI: 10.1038/nbt.3973. View