Multiplexed Quantification of Proteins and Transcripts in Single Cells

Overview

Journal Nat Biotechnol

Specialty Biotechnology

Date 2017 Aug 31

PMID 28854175

Citations 417

Authors

Vanessa M Peterson

Kelvin Xi Zhang

Namit Kumar

Jerelyn Wong

Lixia Li

Douglas C Wilson

Renee Moore

Terrill K McClanahan

Svetlana Sadekova

Joel A Klappenbach

Affiliations

Soon will be listed here.

Abstract

We present a tool to measure gene and protein expression levels in single cells with DNA-labeled antibodies and droplet microfluidics. Using the RNA expression and protein sequencing assay (REAP-seq), we quantified proteins with 82 barcoded antibodies and >20,000 genes in a single workflow. We used REAP-seq to assess the costimulatory effects of a CD27 agonist on human CD8 lymphocytes and to identify and characterize an unknown cell type.

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