Fluorescence Anisotropy As a Temperature-Sensing Molecular Probe Using Fluorescein

Overview

Journal Micromachines (Basel)

Publisher MDPI

Date 2021 Sep 28

PMID 34577751

Citations 3

Authors

Puneet Jain

Takuya Aida

Masahiro Motosuke

Affiliations

Soon will be listed here.

Abstract

Fluorescence anisotropy, a technique to study the folding state of proteins or affinity of ligands, is used in this present work as a temperature sensor, to measure the microfluidic temperature field, by adding fluorophore in the liquid. Fluorescein was used as a temperature-sensing probe, while glycerol-aq. ammonia solution was used as a working fluid. Fluorescence anisotropy of fluorescein was measured by varying various parameters. Apart from this, a comparison of fluorescence anisotropy and fluorescence intensity is also performed to demonstrate the validity of anisotropy to be applied in a microfluidic field with non-uniform liquid thickness. Viscosity dependence and temperature dependence on the anisotropy are also clarified; the results indicate an appropriate selection of relation between molecule size and viscosity is important to obtain a large temperature coefficient in anisotropy. Furthermore, a practical calibration procedure of the apparatus constant is proposed. In addition, the potential of temperature imaging is confirmed by the measurement of temperature distribution under focused laser heating.

Citing Articles

Investigating the Mechanism of Rare-Earth Ion Incorporation into Glass-Ceramic Crystal Phases through Er Ion Probe Characteristics.

Chen Z, Cui W, Ren S, Yang J, Tian J, Xia H Nanomaterials (Basel). 2024; 14(18).

PMID: 39330637 PMC: 11434672. DOI: 10.3390/nano14181479.

A cost-effective approach to measurements of fluorophore temperature sensitivity and temperature change with reasonable accuracy.

Cai M, Sun A, Yan A, Ding Z, Jiang M, Wang C Sci Rep. 2024; 14(1):6823.

PMID: 38514729 PMC: 10957993. DOI: 10.1038/s41598-024-57387-2.

Functioning of a Fluorescein pH-Probe in Aqueous Media: Impact of Temperature and Viscosity.

Surzhikova D, Sukovatyi L, Nemtseva E, Esimbekova E, Slyusareva E Micromachines (Basel). 2023; 14(7).

PMID: 37512752 PMC: 10383544. DOI: 10.3390/mi14071442.

References

Moura-Melo S, Miranda-Castro R, de-Los-Santos-Alvarez N, Miranda-Ordieres A, Dos Santos Junior J, da Silva Fonseca R . A Quantitative PCR-Electrochemical Genosensor Test for the Screening of Biotech Crops. Sensors (Basel). 2017; 17(4). PMC: 5424758. DOI: 10.3390/s17040881. View

Leung W, Pang C, Pang S, Weng S, Lin Y, Chiou Y . High-Sensitivity Dual-Probe Detection of Urinary miR-141 in Cancer Patients via a Modified Screen-Printed Carbon Electrode-Based Electrochemical Biosensor. Sensors (Basel). 2021; 21(9). PMC: 8125155. DOI: 10.3390/s21093183. View

Al-Senani G, Al-Kadhi N . Studies on Adsorption of Fluorescein Dye from Aqueous Solutions Using Wild Herbs. Int J Anal Chem. 2020; 2020:8019274. PMC: 7201707. DOI: 10.1155/2020/8019274. View

Miralles V, Huerre A, Malloggi F, Jullien M . A Review of Heating and Temperature Control in Microfluidic Systems: Techniques and Applications. Diagnostics (Basel). 2016; 3(1):33-67. PMC: 4665581. DOI: 10.3390/diagnostics3010033. View

Loura L, Prieto M . FRET in Membrane Biophysics: An Overview. Front Physiol. 2011; 2:82. PMC: 3216123. DOI: 10.3389/fphys.2011.00082. View

Hochstetter A . Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics. Micromachines (Basel). 2020; 11(5). PMC: 7281269. DOI: 10.3390/mi11050468. View

Vinegoni C, Fumene Feruglio P, Gryczynski I, Mazitschek R, Weissleder R . Fluorescence anisotropy imaging in drug discovery. Adv Drug Deliv Rev. 2018; 151-152:262-288. PMC: 6072632. DOI: 10.1016/j.addr.2018.01.019. View

Niidome T, Akiyama Y, Yamagata M, Kawano T, Mori T, Niidome Y . Poly(ethylene glycol)-modified gold nanorods as a photothermal nanodevice for hyperthermia. J Biomater Sci Polym Ed. 2009; 20(9):1203-15. DOI: 10.1163/156856209X452953. View

Ojha N, Rainey K, Patterson G . Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association. Nat Commun. 2020; 11(1):21. PMC: 6946710. DOI: 10.1038/s41467-019-13843-6. View

10.

Khan T, Price B, Morgan P, Maldonado-Codina C, Dobson C . Cellular fluorescein hyperfluorescence is dynamin-dependent and increased by Tetronic 1107 treatment. Int J Biochem Cell Biol. 2018; 101:54-63. DOI: 10.1016/j.biocel.2018.05.011. View

11.

Ross D, Gaitan M, Locascio L . Temperature measurement in microfluidic systems using a temperature-dependent fluorescent dye. Anal Chem. 2001; 73(17):4117-23. DOI: 10.1021/ac010370l. View

12.

Ali H, Hassan A, Hassan Y, El-Wekil M . One pot fabrication of fluorescein functionalized manganese dioxide for fluorescence "Turn OFF-ON" sensing of hydrogen peroxide in water and cosmetic samples. RSC Adv. 2022; 10(30):17506-17514. PMC: 9053515. DOI: 10.1039/d0ra01980a. View

13.

Yang F, Yang N, Huo X, Xu S . Thermal sensing in fluid at the micro-nano-scales. Biomicrofluidics. 2019; 12(4):041501. PMC: 6404956. DOI: 10.1063/1.5037421. View

14.

Watanabe M, Kakuta N, Mabuchi K, Yamada Y . Micro-thermocouple probe for measurement of cellular thermal responses. Conf Proc IEEE Eng Med Biol Soc. 2007; 2005:4858-61. DOI: 10.1109/IEMBS.2005.1615560. View

15.

Stawska H, Popenda M . Fluorescence Anisotropy Sensor Comprising a Dual Hollow-Core Antiresonant Fiber Polarization Beam Splitter. Sensors (Basel). 2020; 20(11). PMC: 7308924. DOI: 10.3390/s20113321. View

16.

Liu H, Ma L, Ma C, Du J, Wang M, Wang K . Quencher-Free Fluorescence Method for the Detection of Mercury(II) Based on Polymerase-Aided Photoinduced Electron Transfer Strategy. Sensors (Basel). 2016; 16(11). PMC: 5134604. DOI: 10.3390/s16111945. View

17.

Gabbarini V, Rossi R, Ciparisse J, Malizia A, Divizia A, De Filippis P . Laser-induced fluorescence (LIF) as a smart method for fast environmental virological analyses: validation on Picornaviruses. Sci Rep. 2019; 9(1):12598. PMC: 6715700. DOI: 10.1038/s41598-019-49005-3. View

18.

Hoang V, Kaigala G, Backhouse C . Dynamic temperature measurement in microfluidic devices using thermochromic liquid crystals. Lab Chip. 2008; 8(3):484-7. DOI: 10.1039/b713764h. View

19.

Donner J, Thompson S, Kreuzer M, Baffou G, Quidant R . Mapping intracellular temperature using green fluorescent protein. Nano Lett. 2012; 12(4):2107-11. DOI: 10.1021/nl300389y. View

20.

Gradinaru C, Marushchak D, Samim M, Krull U . Fluorescence anisotropy: from single molecules to live cells. Analyst. 2010; 135(3):452-9. DOI: 10.1039/b920242k. View