Serum-Derived Neuronal Exosomal MiRNAs As Biomarkers of Acute Severe Stress

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Sep 28

PMID 34576126

Citations 4

Authors

MinKyoung Sung

Soo-Eun Sung

Kyung-Ku Kang

Joo-Hee Choi

Sijoon Lee

KilSoo Kim

Ju-Hyeon Lim

Gun Woo Lee

Hyo-Deog Rim

Byung-Soo Kim

Seunghee Won

Kyungmin Kim

Seoyoung Jang

Min-Soo Seo

Jungmin Woo

Affiliations

Soon will be listed here.

Abstract

Stress is the physical and psychological tension felt by an individual while adapting to difficult situations. Stress is known to alter the expression of stress hormones and cause neuroinflammation in the brain. In this study, miRNAs in serum-derived neuronal exosomes (nEVs) were analyzed to determine whether differentially expressed miRNAs could be used as biomarkers of acute stress. Specifically, acute severe stress was induced in Sprague-Dawley rats via electric foot-shock treatment. In this acute severe-stress model, time-dependent changes in the expression levels of stress hormones and neuroinflammation-related markers were analyzed. In addition, nEVs were isolated from the serum of control mice and stressed mice at various time points to determine when brain damage was most prominent; this was found to be 7 days after foot shock. Next-generation sequencing was performed to compare neuronal exosomal miRNA at day 7 with the neuronal exosomal miRNA of the control group. From this analysis, 13 upregulated and 11 downregulated miRNAs were detected. These results show that specific miRNAs are differentially expressed in nEVs from an acute severe-stress animal model. Thus, this study provides novel insights into potential stress-related biomarkers.

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