Transport Engineering for Improving the Production and Secretion of Valuable Alkaloids in

Overview

Journal Metab Eng Commun

Specialty Biochemistry

Date 2021 Sep 27

PMID 34567974

Citations 8

Authors

Yasuyuki Yamada

Miya Urui

Hidehiro Oki

Kai Inoue

Haruyuki Matsui

Yoshito Ikeda

Akira Nakagawa

Fumihiko Sato

Hiromichi Minami

Nobukazu Shitan

Affiliations

Soon will be listed here.

Abstract

Microorganisms can be metabolically engineered to produce specialized plant metabolites. However, these methods are limited by low productivity and intracellular accumulation of metabolites. We sought to use transport engineering for producing reticuline, an important intermediate in the alkaloid biosynthetic pathway. In this study, we established a reticuline-producing strain into which the multidrug and toxic compound extrusion transporter AtDTX1 was introduced. AtDTX1 was selected due to its suitable expression in and its reticuline-transport activity. Expression of AtDTX1 enhanced reticuline production by 11-fold, and the produced reticuline was secreted into the medium. AtDTX1 expression also conferred high plasmid stability and resulted in upregulation or downregulation of several genes associated with biological processes, including metabolic pathways for reticuline biosynthesis, leading to the production and secretion of high levels of reticuline. The successful employment of a transporter for alkaloid production suggests that the proposed transport engineering approach may improve the biosynthesis of specialized metabolites metabolic engineering.

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