Transportation Engineering for Enhanced Production of Plant Natural Products in Microbial Cell Factories

Overview

Journal Synth Syst Biotechnol

Specialty Biotechnology

Date 2024 Jul 8

PMID 38974023

Authors

Yimeng Zuo

Minghui Zhao

Yuanwei Gou

Lei Huang

Zhinan Xu

Jiazhang Lian

Affiliations

Soon will be listed here.

Abstract

Plant natural products (PNPs) exhibit a wide range of biological activities and have essential applications in various fields such as medicine, agriculture, and flavors. Given their natural limitations, the production of high-value PNPs using microbial cell factories has become an effective alternative in recent years. However, host metabolic burden caused by its massive accumulation has become one of the main challenges for efficient PNP production. Therefore, it is necessary to strengthen the transmembrane transport process of PNPs. This review introduces the discovery and mining of PNP transporters to directly mediate PNP transmembrane transportation both intracellularly and extracellularly. In addition to transporter engineering, this review also summarizes several auxiliary strategies (such as small molecules, environmental changes, and vesicles assisted transport) for strengthening PNP transportation. Finally, this review is concluded with the applications and future perspectives of transportation engineering in the construction and optimization of PNP microbial cell factories.

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