Origin and Evolution of Transporter Substrate Specificity Within the NPF Family

Overview

Journal Elife

Specialty Biology

Date 2017 Mar 4

PMID 28257001

Citations 51

Authors

Morten Egevang Jorgensen

Deyang Xu

Christoph Crocoll

Heidi Asschenfeldt Ernst

David Ramirez

Mohammed Saddik Motawia

Carl Erik Olsen

Osman Mirza

Hussam Hassan Nour-Eldin

Barbara Ann Halkier

Affiliations

Soon will be listed here.

Abstract

Despite vast diversity in metabolites and the matching substrate specificity of their transporters, little is known about how evolution of transporter substrate specificities is linked to emergence of substrates via evolution of biosynthetic pathways. Transporter specificity towards the recently evolved glucosinolates characteristic of is shown to evolve prior to emergence of glucosinolate biosynthesis. Furthermore, we show that glucosinolate transporters belonging to the ubiquitous NRT1/PTR FAMILY (NPF) likely evolved from transporters of the ancestral cyanogenic glucosides found across more than 2500 species outside of the . Biochemical characterization of orthologs along the phylogenetic lineage from cassava to suggests that alterations in the electrogenicity of the transporters accompanied changes in substrate specificity. Linking the evolutionary path of transporter substrate specificities to that of the biosynthetic pathways, exemplify how transporter substrate specificities originate and evolve as new biosynthesis pathways emerge.

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