Chromosome Painting Provides Insights Into the Genome Structure and Evolution of Sugarcane

Overview

Journal Front Plant Sci

Date 2021 Sep 13

PMID 34512706

Citations 7

Authors

Zhuang Meng

Qinnan Wang

Haris Khurshid

Ghulam Raza

Jinlei Han

Baohua Wang

Kai Wang

Affiliations

Soon will be listed here.

Abstract

The genus is composed of species with high polyploidy and highly varied chromosome numbers, laying a challenge for uncovering its genomic structure and evolution. We developed a chromosome 2 painting (CP2) probe by designing oligonucleotides covering chromosome 2 of (2n = 8x = 64). Fluorescence hybridization (FISH) using this CP2 probe revealed six types of ploidies from twenty clones, including 6x, 8x, 10x, 11x, 12x, and 13x clones. The finding of clones with uneven of ploid suggested that certain clones come from hybridization. It renews our knowledge that is derived from autopolyploidization. Combined with a -specific probe, chromosome 2-derived chromosome or fragments from either or can be identified in sugarcane modern cultivars. We revealed unexpected high level of interspecific recombination from introgressive chromosomes (>50.0%) in cultivars ROC22 and ZZ1, indicating frequent chromosome exchange in cultivars. Intriguingly, we observed interspecific recombination recurring among either homoeologous or non-homoeologous chromosomes in sugarcane cultivars. These results demonstrated that chromosome painting FISH is a powerful tool in the genome dissection of sugarcane and provide new insights into the genome structure and evolution of the complex genus .

Citing Articles

Reconstruction of karyotypic evolution in Saccharum spontaneum species by comparative oligo-FISH mapping.

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A draft chromosome-scale genome assembly of a commercial sugarcane.

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