Development of a Novel Electrochemical Biosensor Based on Carbon Nanofibers-Cobalt Phthalocyanine-Laccase for the Detection of P-Coumaric Acid in Phytoproducts

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Sep 10

PMID 34502203

Citations 9

Authors

Alexandra Virginia Bounegru

Constantin Apetrei

Affiliations

Soon will be listed here.

Abstract

The present paper developed a new enzymatic biosensor whose support is a screen-printed electrode based on carbon nanofibers modified with cobalt phthalocyanine and laccase (CNF-CoPc-Lac/SPE) to determine the p-coumaric acid (PCA) content by cyclic voltammetry and square wave voltammetry. Sensor modification was achieved by the casting and cross-linking technique, using glutaraldehyde as a reticulation agent. The biosensor's response showed the PCA redox processes in a very stable and sensitive manner. The calibration curve was developed for the concentration range of p-coumaric acid of 0.1-202.5 μM, using cyclic voltammetry and chronoamperometry. The biosensor yielded optimal results for the linearity range 0.4-6.4 μM and stood out by low LOD and LOQ values, i.e., 4.83 × 10 M and 1.61 × 10 M, respectively. PCA was successfully determined in three phytoproducts of complex composition. The results obtained by the voltammetric method were compared to the ones obtained by the FTIR method. The amount of p-coumaric acid determined by means of CNF-CoPc-Lac/SPE was close to the one obtained by the standard spectrometric method.

Citing Articles

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References

Kilic I, Yesiloglu Y . Spectroscopic studies on the antioxidant activity of p-coumaric acid. Spectrochim Acta A Mol Biomol Spectrosc. 2013; 115:719-24. DOI: 10.1016/j.saa.2013.06.110. View

Lee S, Mun G, An S, Boo Y . Evidence for the association of peroxidases with the antioxidant effect of p-coumaric acid in endothelial cells exposed to high glucose plus arachidonic acid. BMB Rep. 2009; 42(9):561-7. DOI: 10.5483/bmbrep.2009.42.9.561. View

Bounegru A, Apetrei C . Development of a Novel Electrochemical Biosensor Based on Carbon Nanofibers-Gold Nanoparticles-Tyrosinase for the Detection of Ferulic Acid in Cosmetics. Sensors (Basel). 2020; 20(23). PMC: 7727797. DOI: 10.3390/s20236724. View

Kaneko T, Thi T, Shi D, Akashi M . Environmentally degradable, high-performance thermoplastics from phenolic phytomonomers. Nat Mater. 2006; 5(12):966-70. DOI: 10.1038/nmat1778. View

Lee I, Boyce M, Breadmore M . A rapid quantitative determination of phenolic acids in Brassica oleracea by capillary zone electrophoresis. Food Chem. 2012; 127(2):797-801. DOI: 10.1016/j.foodchem.2011.01.015. View

Nazari M, Kashanian S, Rafipour R . Laccase immobilization on the electrode surface to design a biosensor for the detection of phenolic compound such as catechol. Spectrochim Acta A Mol Biomol Spectrosc. 2015; 145:130-138. DOI: 10.1016/j.saa.2015.01.126. View

Boo Y . -Coumaric Acid as An Active Ingredient in Cosmetics: A Review Focusing on its Antimelanogenic Effects. Antioxidants (Basel). 2019; 8(8). PMC: 6720745. DOI: 10.3390/antiox8080275. View

Kim W, Lim D, Kim J . p-Coumaric Acid, a Major Active Compound of Bambusae Caulis in Taeniam, Suppresses Cigarette Smoke-Induced Pulmonary Inflammation. Am J Chin Med. 2018; 46(2):407-421. DOI: 10.1142/S0192415X18500209. View

Cha H, Lee S, Lee J, Park J . Protective effects of p-coumaric acid against acetaminophen-induced hepatotoxicity in mice. Food Chem Toxicol. 2018; 121:131-139. DOI: 10.1016/j.fct.2018.08.060. View

10.

Marti R, Valcarcel M, Herrero-Martinez J, Cebolla-Cornejo J, Rosello S . Simultaneous determination of main phenolic acids and flavonoids in tomato by micellar electrokinetic capillary electrophoresis. Food Chem. 2016; 221:439-446. DOI: 10.1016/j.foodchem.2016.10.105. View

11.

Kawaguchi H, Katsuyama Y, Danyao D, Kahar P, Nakamura-Tsuruta S, Teramura H . Caffeic acid production by simultaneous saccharification and fermentation of kraft pulp using recombinant Escherichia coli. Appl Microbiol Biotechnol. 2017; 101(13):5279-5290. DOI: 10.1007/s00253-017-8270-0. View

12.

Zhao S, Jones J, Lachance D, Bhan N, Khalidi O, Venkataraman S . Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014; 28:43-53. DOI: 10.1016/j.ymben.2014.12.002. View

13.

Peng J, Zheng T, Liang Y, Duan L, Zhang Y, Wang L . -Coumaric Acid Protects Human Lens Epithelial Cells against Oxidative Stress-Induced Apoptosis by MAPK Signaling. Oxid Med Cell Longev. 2018; 2018:8549052. PMC: 5914090. DOI: 10.1155/2018/8549052. View

14.

Giardina P, Faraco V, Pezzella C, Piscitelli A, Vanhulle S, Sannia G . Laccases: a never-ending story. Cell Mol Life Sci. 2009; 67(3):369-85. PMC: 11115910. DOI: 10.1007/s00018-009-0169-1. View

15.

Nijkamp K, Westerhof R, Ballerstedt H, de Bont J, Wery J . Optimization of the solvent-tolerant Pseudomonas putida S12 as host for the production of p-coumarate from glucose. Appl Microbiol Biotechnol. 2006; 74(3):617-24. DOI: 10.1007/s00253-006-0703-0. View

16.

Li M, Kildegaard K, Chen Y, Rodriguez A, Borodina I, Nielsen J . De novo production of resveratrol from glucose or ethanol by engineered Saccharomyces cerevisiae. Metab Eng. 2015; 32:1-11. DOI: 10.1016/j.ymben.2015.08.007. View

17.

Oliveira-Neto J, Rezende S, Reis C, Benjamin S, Rocha M, Gil E . Electrochemical behavior and determination of major phenolic antioxidants in selected coffee samples. Food Chem. 2015; 190:506-512. DOI: 10.1016/j.foodchem.2015.05.104. View

18.

Ribeiro F, Barroso M, Morais S, Viswanathan S, de Lima-Neto P, Correia A . Simple laccase-based biosensor for formetanate hydrochloride quantification in fruits. Bioelectrochemistry. 2013; 95:7-14. DOI: 10.1016/j.bioelechem.2013.09.005. View

19.

Chaiyo S, Mehmeti E, Siangproh W, Hoang T, Nguyen H, Chailapakul O . Non-enzymatic electrochemical detection of glucose with a disposable paper-based sensor using a cobalt phthalocyanine-ionic liquid-graphene composite. Biosens Bioelectron. 2017; 102:113-120. DOI: 10.1016/j.bios.2017.11.015. View

20.

Vanholme R, Storme V, Vanholme B, Sundin L, Christensen J, Goeminne G . A systems biology view of responses to lignin biosynthesis perturbations in Arabidopsis. Plant Cell. 2012; 24(9):3506-29. PMC: 3480285. DOI: 10.1105/tpc.112.102574. View