Carbon Nanomaterials (CNMs) and Enzymes: From Nanozymes to CNM-Enzyme Conjugates and Biodegradation

Overview

Journal Materials (Basel)

Publisher MDPI

Date 2022 Feb 15

PMID 35160982

Authors

Petr Rozhin

Jada Abdel Monem Gamal

Silvia Giordani

Silvia Marchesan

Affiliations

Soon will be listed here.

Abstract

Carbon nanomaterials (CNMs) and enzymes differ significantly in terms of their physico-chemical properties-their handling and characterization require very different specialized skills. Therefore, their combination is not trivial. Numerous studies exist at the interface between these two components-especially in the area of sensing-but also involving biofuel cells, biocatalysis, and even biomedical applications including innovative therapeutic approaches and theranostics. Finally, enzymes that are capable of biodegrading CNMs have been identified, and they may play an important role in controlling the environmental fate of these structures after their use. CNMs' widespread use has created more and more opportunities for their entry into the environment, and thus it becomes increasingly important to understand how to biodegrade them. In this concise review, we will cover the progress made in the last five years on this exciting topic, focusing on the applications, and concluding with future perspectives on research combining carbon nanomaterials and enzymes.

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References

Xue S, Zhang T, Wang X, Zhang Q, Huang S, Zhang L . Cu,Zn Dopants Boost Electron Transfer of Carbon Dots for Antioxidation. Small. 2021; 17(31):e2102178. DOI: 10.1002/smll.202102178. View

He Z, Cai Y, Yang Z, Li P, Lei H, Liu W . A dual-signal readout enzyme-free immunosensor based on hybridization chain reaction-assisted formation of copper nanoparticles for the detection of microcystin-LR. Biosens Bioelectron. 2018; 126:151-159. DOI: 10.1016/j.bios.2018.10.033. View

Cheng N, Li J, Liu D, Lin Y, Du D . Single-Atom Nanozyme Based on Nanoengineered Fe-N-C Catalyst with Superior Peroxidase-Like Activity for Ultrasensitive Bioassays. Small. 2019; 15(48):e1901485. DOI: 10.1002/smll.201901485. View

Liu X, Huang D, Lai C, Qin L, Zeng G, Xu P . Peroxidase-Like Activity of Smart Nanomaterials and Their Advanced Application in Colorimetric Glucose Biosensors. Small. 2019; 15(17):e1900133. DOI: 10.1002/smll.201900133. View

Whitlow J, Pacelli S, Paul A . Multifunctional nanodiamonds in regenerative medicine: Recent advances and future directions. J Control Release. 2017; 261:62-86. PMC: 5560434. DOI: 10.1016/j.jconrel.2017.05.033. View

Liu J, Li R, Yang B . Carbon Dots: A New Type of Carbon-Based Nanomaterial with Wide Applications. ACS Cent Sci. 2020; 6(12):2179-2195. PMC: 7760469. DOI: 10.1021/acscentsci.0c01306. View

Rahamathulla M, Bhosale R, Osmani R, Mahima K, Johnson A, Hani U . Carbon Nanotubes: Current Perspectives on Diverse Applications in Targeted Drug Delivery and Therapies. Materials (Basel). 2021; 14(21). PMC: 8588285. DOI: 10.3390/ma14216707. View

Wu G, Gao Y, Zhao D, Ling P, Gao F . Methanol/Oxygen Enzymatic Biofuel Cell Using Laccase and NAD-Dependent Dehydrogenase Cascades as Biocatalysts on Carbon Nanodots Electrodes. ACS Appl Mater Interfaces. 2017; 9(46):40978-40986. DOI: 10.1021/acsami.7b12295. View

Sharma V, Tiwari P, Mobin S . Sustainable carbon-dots: recent advances in green carbon dots for sensing and bioimaging. J Mater Chem B. 2020; 5(45):8904-8924. DOI: 10.1039/c7tb02484c. View

10.

Liu Y, Yan B, Winkler D, Fu J, Zhang A . Competitive Inhibition Mechanism of Acetylcholinesterase without Catalytic Active Site Interaction: Study on Functionalized C Nanoparticles via in Vitro and in Silico Assays. ACS Appl Mater Interfaces. 2017; 9(22):18626-18638. DOI: 10.1021/acsami.7b05459. View

11.

Yang F, Wang M, Zhang D, Yang J, Zheng M, Li Y . Chirality Pure Carbon Nanotubes: Growth, Sorting, and Characterization. Chem Rev. 2020; 120(5):2693-2758. DOI: 10.1021/acs.chemrev.9b00835. View

12.

Campuzano S, Yanez-Sedeno P, Pingarron J . Carbon Dots and Graphene Quantum Dots in Electrochemical Biosensing. Nanomaterials (Basel). 2019; 9(4). PMC: 6523669. DOI: 10.3390/nano9040634. View

13.

Bei H, Yang Y, Zhang Q, Tian Y, Luo X, Yang M . Graphene-Based Nanocomposites for Neural Tissue Engineering. Molecules. 2019; 24(4). PMC: 6413135. DOI: 10.3390/molecules24040658. View

14.

Wang H, Li P, Yu D, Zhang Y, Wang Z, Liu C . Unraveling the Enzymatic Activity of Oxygenated Carbon Nanotubes and Their Application in the Treatment of Bacterial Infections. Nano Lett. 2018; 18(6):3344-3351. DOI: 10.1021/acs.nanolett.7b05095. View

15.

Yang M, Wu X, Hu X, Wang K, Zhang C, Gyimah E . Electrochemical immunosensor based on Ag-dependent CTAB-AuNPs for ultrasensitive detection of sulfamethazine. Biosens Bioelectron. 2019; 144:111643. DOI: 10.1016/j.bios.2019.111643. View

16.

Anwar S, Ding H, Xu M, Hu X, Li Z, Wang J . Recent Advances in Synthesis, Optical Properties, and Biomedical Applications of Carbon Dots. ACS Appl Bio Mater. 2022; 2(6):2317-2338. DOI: 10.1021/acsabm.9b00112. View

17.

Xu M, Xing S, Zhao Y, Zhao C . Peptide nucleic acid-assisted colorimetric detection of single-nucleotide polymorphisms based on the intrinsic peroxidase-like activity of hemin-carbon nanotube nanocomposites. Talanta. 2021; 232:122420. DOI: 10.1016/j.talanta.2021.122420. View

18.

Barzegar A, Naghizadeh E, Zakariazadeh M, Azamat J . Molecular Dynamics Simulation Study of the HIV-1 Protease Inhibit ion Using Fullerene and New Fullerene Derivatives of Carbon Nanostructures. Mini Rev Med Chem. 2016; 17(7):633-647. DOI: 10.2174/1389557516666160609080157. View

19.

Liu Q, Ma C, Liu X, Wei Y, Mao C, Zhu J . A novel electrochemiluminescence biosensor for the detection of microRNAs based on a DNA functionalized nitrogen doped carbon quantum dots as signal enhancers. Biosens Bioelectron. 2017; 92:273-279. DOI: 10.1016/j.bios.2017.02.027. View

20.

Lv S, Li Y, Zhang K, Lin Z, Tang D . Carbon Dots/g-CN Nanoheterostructures-Based Signal-Generation Tags for Photoelectrochemical Immunoassay of Cancer Biomarkers Coupling with Copper Nanoclusters. ACS Appl Mater Interfaces. 2017; 9(44):38336-38343. DOI: 10.1021/acsami.7b13272. View