Method of Transmission Filters to Measure Emission Spectra in Strongly Scattering Media

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2021 Aug 30

PMID 34457378

Citations 2

Authors

Belen Torrado

Alexander Dvornikov

Enrico Gratton

Affiliations

Soon will be listed here.

Abstract

We describe a method based on a pair of transmission filters placed in the emission path of a microscope to resolve the emission wavelength of every point in an image. The method can be applied to any type of imaging device that provides the light in the wavelength transmission range of the filters. Unique characteristics of the filter approach are that the light does not need to be collimated and the wavelength response does not depend on the scattering of the sample or tissue. The pair of filters are used to produce the spectral phasor of the transmitted light, which is sufficient to perform spectral deconvolution over a broad wavelength range. The method is sensitive enough to distinguish free and protein-bound NADH and can be used in metabolic studies.

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