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A Deep Tissue Fluorescence Imaging System with Enhanced SHG Detection Capabilities

Overview
Journal Microsc Res Tech
Specialty Radiology
Date 2014 Mar 11
PMID 24610799
Citations 18
Authors
Viera Crosignani
Sohail Jahid
Alexander S Dvornikov
Enrico Gratton
Affiliations
Soon will be listed here.
Abstract

We describe a novel two-photon fluorescence microscopy system capable of producing high-quality second harmonic generation (SHG) images in thick turbid media by using an innovative detection system. This novel detection system is capable of detecting photons from a very large surface area. This system has proven effective in providing images of thick turbid samples, both biological and artificial. Due to its transmission detection geometry, the system is particularly suitable for detecting SHG signals, which are generally forward directed. In this article, we present comparative data acquired simultaneously on the same sample with the forward and epidetection schemes.

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