OIP5-AS1 Contributes to the Development in Endometrial Carcinoma Cells by Targeting MiR-152-3p to Up-regulate SLC7A5

Overview

Journal Cancer Cell Int

Publisher Biomed Central

Date 2021 Aug 22

PMID 34419049

Citations 6

Authors

Minglin Liang

Hongbo Wang

Cong Liu

Tao Lei

Jie Min

Affiliations

Soon will be listed here.

Abstract

Background: Endometrial carcinoma (EC) is one common gynecological tumor, threatening physical and psychological health of females. Huge amount of essays indicated that long non-coding RNAs (lncRNAs) were widely reported to serve as a crucial regulator in the biological movements among multiple carcinomas, including EC.

Methods: RT-qPCR was implemented to detect the expression of target genes. Loss/gain-of-function experiments certified the impacts of OIP5-AS1 and miR-152-3p on EC cell progression.

Results: Data of this research suggested that powerful expression of OIP5-AS1 was discovered in EC cell lines. Loss/gain-of-function assays inferred that OIP5-AS1 promoted proliferative, migratory and invasive abilities, and Epithelial-Mesenchymal Transition (EMT). In addition, we identified miR-152-3p expression was negatively modulated by OIP5-AS1. OIP5-AS1 accelerated the development of EC cells via downregulating miR-152-3p expression. SLC7A5 was selected out as a downstream target of miR-152-3p. The competing relationship between OIP5-AS1 and SLC7A5 was corroborated by luciferase reporter assay. Eventually, the results of rescue assays indicated that SLC7A5 overexpression could restore the impacts of OIP5-AS1 ablation on the progression of EC cells.

Conclusion: Our research confirmed that OIP5-AS1 propeled the development of EC cells through targeting miR-152-3p/SLC7A5. OIP5-AS1 could be utilized as a target for EC treatment.

Citing Articles

m6A- and m5C- modified lncRNAs orchestrate the prognosis in cutaneous melanoma and m6A- modified LINC00893 regulates cutaneous melanoma cell metastasis.

Shi H, Tian C, Wu M, Ma L, Sun J, Chen H Skin Res Technol. 2024; 30(7):e13842.

PMID: 38965799 PMC: 11224130. DOI: 10.1111/srt.13842.

Baicalein improves the chemoresistance of ovarian cancer through regulation of CirSLC7A6.

Li S, Yi Z, Li M, Zhu Z J Ovarian Res. 2023; 16(1):212.

PMID: 37940982 PMC: 10631197. DOI: 10.1186/s13048-023-01285-0.

The Involvement of Long Non-Coding RNAs in Glutamine-Metabolic Reprogramming and Therapeutic Resistance in Cancer.

Roh J, Im M, Chae Y, Kang J, Kim W Int J Mol Sci. 2022; 23(23).

PMID: 36499136 PMC: 9738059. DOI: 10.3390/ijms232314808.

Circ_0000284 facilitates the growth, metastasis and glycolysis of intrahepatic cholangiocarcinoma through miR-152-3p-mediated PDK1 expression.

Sun J, Feng M, Zou H, Mao Y, Yu W Histol Histopathol. 2022; 38(10):1129-1143.

PMID: 36331285 DOI: 10.14670/HH-18-544.

LncRNA OIP5-AS1 modulates the proliferation and apoptosis of Jurkat cells by sponging miR-181c-5p to regulate IL-7 expression in myasthenia gravis.

Wang X, Zhang H, Lu X, Li S, Kong X, Liu L PeerJ. 2022; 10:e13454.

PMID: 35602889 PMC: 9121865. DOI: 10.7717/peerj.13454.

References

Zou Y, Yao S, Chen X, Liu D, Wang J, Yuan X . LncRNA OIP5-AS1 regulates radioresistance by targeting DYRK1A through miR-369-3p in colorectal cancer cells. Eur J Cell Biol. 2018; 97(5):369-378. DOI: 10.1016/j.ejcb.2018.04.005. View

Wang Y, Zhu Y, Lv P, Li L . The role of miR-21 in proliferation and invasion capacity of human tongue squamous cell carcinoma in vitro. Int J Clin Exp Pathol. 2015; 8(5):4555-63. PMC: 4503017. View

Saadeh F, Langhe R, Galvin D, O Toole S, ODonnell D, Gleeson N . Procoagulant activity in gynaecological cancer patients; the effect of surgery and chemotherapy. Thromb Res. 2016; 139:135-41. DOI: 10.1016/j.thromres.2016.01.027. View

Song H, He P, Shao T, Li Y, Li J, Zhang Y . Long non-coding RNA XIST functions as an oncogene in human colorectal cancer by targeting miR-132-3p. J BUON. 2017; 22(3):696-703. View

Loukovaara M, Luomaranta A, Leminen A . [Treatment of endometrial carcinoma]. Duodecim. 2015; 130(22-23):2348-55. View

Lopez-Janeiro A, Ruz-Caracuel I, Ramon-Patino J, de Los Rios V, Villalba Esparza M, Berjon A . Proteomic Analysis of Low-Grade, Early-Stage Endometrial Carcinoma Reveals New Dysregulated Pathways Associated with Cell Death and Cell Signaling. Cancers (Basel). 2021; 13(4). PMC: 7917913. DOI: 10.3390/cancers13040794. View

Luan W, Li R, Liu L, Ni X, Shi Y, Xia Y . Long non-coding RNA HOTAIR acts as a competing endogenous RNA to promote malignant melanoma progression by sponging miR-152-3p. Oncotarget. 2017; 8(49):85401-85414. PMC: 5689618. DOI: 10.18632/oncotarget.19910. View

Li Z, Gao B, Hao S, Tian W, Chen Y, Wang L . Knockdown of lncRNA-PANDAR suppresses the proliferation, cell cycle and promotes apoptosis in thyroid cancer cells. EXCLI J. 2017; 16:354-362. PMC: 5427478. DOI: 10.17179/excli2017-113. View

Deng J, Deng H, Liu C, Liang Y, Wang S . Long non-coding RNA OIP5-AS1 functions as an oncogene in lung adenocarcinoma through targeting miR-448/Bcl-2. Biomed Pharmacother. 2017; 98:102-110. DOI: 10.1016/j.biopha.2017.12.031. View

10.

Wang M, Sun X, Yang Y, Jiao W . Long non-coding RNA OIP5-AS1 promotes proliferation of lung cancer cells and leads to poor prognosis by targeting miR-378a-3p. Thorac Cancer. 2018; 9(8):939-949. PMC: 6068457. DOI: 10.1111/1759-7714.12767. View

11.

Drissennek L, Baron C, Brouillet S, Entezami F, Hamamah S, Haouzi D . Endometrial miRNome profile according to the receptivity status and implantation failure. Hum Fertil (Camb). 2020; 25(2):356-368. DOI: 10.1080/14647273.2020.1807065. View

12.

Ge S, Wang D, Kong Q, Gao W, Sun J . Function of miR-152 as a Tumor Suppressor in Human Breast Cancer by Targeting PIK3CA. Oncol Res. 2017; 25(8):1363-1371. PMC: 7841119. DOI: 10.3727/096504017X14878536973557. View

13.

Li Z, Wu X, Gu L, Shen Q, Luo W, Deng C . Long non-coding RNA ATB promotes malignancy of esophageal squamous cell carcinoma by regulating miR-200b/Kindlin-2 axis. Cell Death Dis. 2017; 8(6):e2888. PMC: 5520904. DOI: 10.1038/cddis.2017.245. View

14.

Bartosch C, Pires M, Jeronimo C, Lopes J . The role of pathology in the management of patients with endometrial carcinoma. Future Oncol. 2017; 13(11):1003-1020. DOI: 10.2217/fon-2016-0570. View

15.

Dai J, Xu L, Hu X, Han G, Jiang H, Sun H . Long noncoding RNA OIP5-AS1 accelerates CDK14 expression to promote osteosarcoma tumorigenesis via targeting miR-223. Biomed Pharmacother. 2018; 106:1441-1447. DOI: 10.1016/j.biopha.2018.07.109. View

16.

Quinn J, Chang H . Unique features of long non-coding RNA biogenesis and function. Nat Rev Genet. 2015; 17(1):47-62. DOI: 10.1038/nrg.2015.10. View

17.

Zhang Z, Liu X, Xu H, Li P, Zeng T, Du W . LINC01170 promotes the progression of endometrial carcinoma by activating the AKT pathway. J BUON. 2019; 23(6):1745-1752. View

18.

Yue Z, Shen J, Huang Q, Qin Y, Li X, Liu G . [MiR-135b promotes proliferation of endometrial carcinoma cells by targeting FOXO1]. Nan Fang Yi Ke Da Xue Xue Bao. 2016; 36(5):675-80. View

19.

Mishra S, Yadav T, Rani V . Exploring miRNA based approaches in cancer diagnostics and therapeutics. Crit Rev Oncol Hematol. 2015; 98:12-23. DOI: 10.1016/j.critrevonc.2015.10.003. View

20.

Liu Q, Guo X, Que S, Yang X, Fan H, Liu M . LncRNA RSU1P2 contributes to tumorigenesis by acting as a ceRNA against let-7a in cervical cancer cells. Oncotarget. 2016; 8(27):43768-43781. PMC: 5546439. DOI: 10.18632/oncotarget.10844. View