Clog-free High-throughput Microfluidic Cell Isolation with Multifunctional Microposts

Overview

Journal Sci Rep

Specialty Science

Date 2021 Aug 18

PMID 34404819

Citations 4

Authors

Dilip Venugopal

Nanda Kasani

Yariswamy Manjunath

Guangfu Li

Jussuf T Kaifi

Jae W Kwon

Affiliations

Soon will be listed here.

Abstract

Microfluidics have been applied to filtration of rare tumor cells from the blood as liquid biopsies. Processing is highly limited by low flow rates and device clogging due to a single function of fluidic paths. A novel method using multifunctional hybrid functional microposts was developed. A swift by-passing route for non-tumor cells was integrated to prevent very common clogging problems. Performance was characterized using microbeads (10 µm) and human cancer cells that were spiked in human blood. Design-I showed a capture efficiency of 96% for microbeads and 87% for cancer cells at 1 ml/min flow rate. An improved Design-II presented a higher capture efficiency of 100% for microbeads and 96% for cancer cells. Our method of utilizing various microfluidic functions of separation, bypass and capture has successfully guaranteed highly efficient separation of rare cells from biological fluids.

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