Modulation of Rotation-induced Lift Force for Cell Filtration in a Low Aspect Ratio Microchannel

Overview

Journal Biomicrofluidics

Publisher American Institute of Physics

Specialty Biomedical Engineering

Date 2014 Nov 8

PMID 25379097

Citations 8

Authors

Jian Zhou

Premkumar Vummidi Giridhar

Susan Kasper

Ian Papautsky

Affiliations

Soon will be listed here.

Abstract

Cell filtration is a critical step in sample preparation in many bioapplications. Herein, we report on a simple, filter-free, microfluidic platform based on hydrodynamic inertial migration. Our approach builds on the concept of two-stage inertial migration which permits precise prediction of microparticle position within the microchannel. Our design manipulates equilibrium positions of larger microparticles by modulating rotation-induced lift force in a low aspect ratio microchannel. Here, we demonstrate filtration of microparticles with extreme efficiency (>99%). Using multiple prostate cell lines (LNCaP and human prostate epithelial tumor cells), we show filtration from spiked blood, with 3-fold concentration and >83% viability. Results of a proliferation assay show normal cell division and suggest no negative effects on intrinsic properties. Considering the planar low-aspect-ratio structure and predictable focusing, we envision promising applications and easy integration with existing lab-on-a-chip systems.

Citing Articles

Viscoelastic microfluidics: progress and challenges.

Zhou J, Papautsky I Microsyst Nanoeng. 2021; 6:113.

PMID: 34567720 PMC: 8433399. DOI: 10.1038/s41378-020-00218-x.

Mapping inertial migration in the cross section of a microfluidic channel with high-speed imaging.

Zhou J, Peng Z, Papautsky I Microsyst Nanoeng. 2021; 6:105.

PMID: 34567714 PMC: 8433405. DOI: 10.1038/s41378-020-00217-y.

Clog-free high-throughput microfluidic cell isolation with multifunctional microposts.

Venugopal D, Kasani N, Manjunath Y, Li G, Kaifi J, Kwon J Sci Rep. 2021; 11(1):16685.

PMID: 34404819 PMC: 8370995. DOI: 10.1038/s41598-021-94123-6.

Resolving dynamics of inertial migration in straight and curved microchannels by direct cross-sectional imaging.

Zhou J, Papautsky I Biomicrofluidics. 2021; 15(1):014101.

PMID: 33425090 PMC: 7785325. DOI: 10.1063/5.0032653.

Label-free microfluidic sorting of microparticles.

Zhou J, Mukherjee P, Gao H, Luan Q, Papautsky I APL Bioeng. 2019; 3(4):041504.

PMID: 31832577 PMC: 6906121. DOI: 10.1063/1.5120501.

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