Image-Based Analysis of Mitochondrial Area and Counting from Adult Mouse Dopaminergic Neurites

Overview

Journal Bio Protoc

Specialty Biology

Date 2021 Aug 16

PMID 34395770

Authors

Nadee Nissanka

Carlos T Moraes

Milena Pinto

Affiliations

Soon will be listed here.

Abstract

Mitochondria form dynamic cytoplasmic networks which undergo morphological changes in order to adapt to cellular stresses and signals. These changes can include alterations in size and number within a given cell. Analysis of the whole network can be a useful metric to assess overall mitochondrial health, particularly in neurons, which are highly sensitive to mitochondrial dysfunction. Here we describe a method which combines immunofluorescence and computerized image analysis to measure mitochondrial morphology (quantification of number, density, and area) in dopaminergic neurites of mice expressing mitochondrially-targeted eYFP.

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