Pleiotropic Roles of Ras GTPases in the Nematode-trapping Fungus Identified Through Multi-omics Analyses

Overview

Journal iScience

Publisher Cell Press

Date 2021 Aug 2

PMID 34337364

Citations 22

Authors

Le Yang

Xuemei Li

Meihua Xie

Na Bai

Jiangliu Yang

Kexin Jiang

Ke-Qin Zhang

Jinkui Yang

Affiliations

Soon will be listed here.

Abstract

The nematode-trapping fungi are ideal agents for controlling pathogenic nematodes. is a representative species of the same, producing traps for nematode predation. Here, three orthologous Ras GTPases (Ras2, Ras3, and Rheb) were characterized in . Our results indicate that they play pleiotropic roles in regulating the mycelial growth, conidiation, stress resistance, and pathogenicity of Furthermore, deletion of and significantly affected the mitochondrial activity, reactive oxygen species levels, lipid storage, and autophagy. Transcriptome analyses of Δ mutant revealed that many repressed genes were associated with signal transduction, energy production, and carbohydrate transport and metabolism. Moreover, metabolic profile analyses showed that AoRas2 and AoRheb affect the biosynthesis of secondary metabolites in . Collectively, these findings provide an in-depth insight into the essential roles of Ras GTPases in vegetative growth, development, and pathogenicity and highlight their importance in the lifestyle switch of the nematode-trapping fungi.

Citing Articles

The Hog1-Nmd5 signaling pathway regulates asexual development, lipid metabolism, stress response, trap morphogenesis, and secondary metabolism of .

Zhao N, Liu Q, Zhu M, Zhu L, Yang J Virulence. 2025; 16(1):2468294.

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Functional Characterization of Ao4g24: An Uncharacterized Gene Involved in Conidiation, Trap Formation, Stress Response, and Secondary Metabolism in .

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AoPrdx2 Regulates Oxidative Stress, Reactive Oxygen Species, Trap Formation, and Secondary Metabolism in .

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Identification of a transcription factor AoMsn2 of the Hog1 signaling pathway contributes to fungal growth, development and pathogenicity in Arthrobotrys oligospora.

Liu Q, Jiang K, Duan S, Zhao N, Shen Y, Zhu L J Adv Res. 2024; 68:1-15.

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PMID: 37779704 PMC: 10537225. DOI: 10.3389/fmicb.2023.1235283.

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