Chromosomes Conformation Capture Coupled with Next-Generation Sequencing (Hi-C) in Plasmodium Falciparum

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2021 Jul 27

PMID 34313981

Citations 3

Authors

Mohit Kumar Gupta

Todd Lenz

Karine G Le Roch

Affiliations

Soon will be listed here.

Abstract

Over the last decades, novel methods have been developed to study the role of chromosome positioning within the nucleus may play in gene regulation. Established proximity ligation-based chromosome conformation capture (3C) techniques such as Hi-C have revealed the existence of chromosome territories, functional nuclear landmarks, and topologically associating domains (TAPs) in most eukaryotic organisms. Adaptation of these methods in apicomplexan parasites has recently uncovered new aspects of 3D genome biology in virulence factors. Here, we describe the Hi-C protocol in the human malaria parasite, Plasmodium falciparum. This method can determine the genome organization in malaria parasites and its role in gene regulation and virulence.

Citing Articles

MORC protein regulates chromatin accessibility and transcriptional repression in the human malaria parasite, .

Chahine Z, Gupta M, Lenz T, Hollin T, Abel S, Banks C Elife. 2024; 12.

PMID: 39636094 PMC: 11620747. DOI: 10.7554/eLife.92499.

DNA-binding protein PfAP2-P regulates parasite pathogenesis during malaria parasite blood stages.

Subudhi A, Green J, Satyam R, Salunke R, Lenz T, Shuaib M Nat Microbiol. 2023; 8(11):2154-2169.

PMID: 37884813 PMC: 10627835. DOI: 10.1038/s41564-023-01497-6.

MORC protein regulates chromatin accessibility and transcriptional repression in the human malaria parasite, .

Chahine Z, Gupta M, Lenz T, Hollin T, Abel S, Banks C bioRxiv. 2023; .

PMID: 37745554 PMC: 10515874. DOI: 10.1101/2023.09.11.557253.

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