Extraction and Quantitative Analysis of Goat Milk Oligosaccharides: Composition, Variation, Associations, and 2'-FL Variability

Overview

Journal J Agric Food Chem

Specialties Chemistry
Nutritional Sciences

Date 2021 Jul 7

PMID 34232652

Citations 3

Authors

Anastasia Chrysovalantou Chatziioannou

Eric Benjamins

Linette Pellis

Alfred Haandrikman

Lubbert Dijkhuizen

Sander S van Leeuwen

Affiliations

Soon will be listed here.

Abstract

Human milk oligosaccharides (hMOS) are associated with health benefits for newborns. We studied the composition of goat MOS (gMOS) from colostrum up to the 9th month of lactation to conceive an overview of the structures present and their fate. Potential correlations with factors such as age, parity, and lifetime milk production were examined. An effective method for gMOS extraction and ultra-high-performance liquid chromatography coupled to fluorescence detection (UPLC-FLD) analysis was established, following 2-aminobenzamide gMOS labeling. Considerable biological variability was highlighted among the 12 quantified gMOS and the 9 non-quantified structures in the individual milk samples. Most characteristic, 2'-fucosyllactose was present in 73.7% of the milk samples analyzed, suggesting the possibility of a secretor/non-secretor goat genotype, similar to humans. Contributing factors to the observed biological variability were goat age, parity, lifetime milk production, and the kids' sex. The results significantly contribute to the current understanding of (variations in) gMOS composition.

Citing Articles

Human Milk Oligosaccharides: Decoding Their Structural Variability, Health Benefits, and the Evolution of Infant Nutrition.

Duman H, Bechelany M, Karav S Nutrients. 2025; 17(1.

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Development and validation of a versatile analytical method for absolute quantification of seven oligosaccharides in human, bovine, and goat milk.

Urrutia-Baca V, Chuck-Hernandez C, Gutierrez-Uribe J, Ramos-Parra P, Licona-Cassani C Heliyon. 2023; 9(11):e22475.

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Biological Relevance of Goat Milk Oligosaccharides to Infant Health.

van der Toorn M, Chatziioannou A, Pellis L, Haandrikman A, van der Zee L, Dijkhuizen L J Agric Food Chem. 2023; 71(38):13935-13949.

PMID: 37691562 PMC: 10540210. DOI: 10.1021/acs.jafc.3c02194.

Characterization of Bioactive Sialyl Oligosaccharides Separated from Colostrum of Indonesia Dairy Goat.

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