Maternal and Infant Factors Associated with Human Milk Oligosaccharides Concentrations According to Secretor and Lewis Phenotypes

Overview

Journal Nutrients

Date 2019 Jun 20

PMID 31212920

Citations 43

Authors

Karina M Tonon

Mauro B de Morais

Ana Cristina F V Abrao

Antonio Miranda

Tania B Morais

Affiliations

Soon will be listed here.

Abstract

Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants. HMOs composition is very individualized and differences in HMOs concentrations may affect the infant's health. HMOs variability can be partially explained by the activity of Secretor () and Lewis () genes in the mother, but non-genetic maternal factors may also be involved. In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated. HMOs concentrations presented a high variability even in women with the same phenotype and associations with maternal allergic disease, time postpartum and with infant's weight, weight gain and sex. Overall, we present unprecedented data on HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations. Differences in HMOs composition attributed to maternal phenotype do not impact infant growth, but higher concentrations of specific HMOs may protect against excessive weight gain.

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References

Chen X . Human Milk Oligosaccharides (HMOS): Structure, Function, and Enzyme-Catalyzed Synthesis. Adv Carbohydr Chem Biochem. 2015; 72:113-90. PMC: 9235823. DOI: 10.1016/bs.accb.2015.08.002. View

Jantscher-Krenn E, Zherebtsov M, Nissan C, Goth K, Guner Y, Naidu N . The human milk oligosaccharide disialyllacto-N-tetraose prevents necrotising enterocolitis in neonatal rats. Gut. 2011; 61(10):1417-25. PMC: 3909680. DOI: 10.1136/gutjnl-2011-301404. View

Azad M, Robertson B, Atakora F, Becker A, Subbarao P, Moraes T . Human Milk Oligosaccharide Concentrations Are Associated with Multiple Fixed and Modifiable Maternal Characteristics, Environmental Factors, and Feeding Practices. J Nutr. 2018; 148(11):1733-1742. DOI: 10.1093/jn/nxy175. View

Ninonuevo M, Park Y, Yin H, Zhang J, Ward R, Clowers B . A strategy for annotating the human milk glycome. J Agric Food Chem. 2006; 54(20):7471-80. DOI: 10.1021/jf0615810. View

Kobata A . Structures and application of oligosaccharides in human milk. Proc Jpn Acad Ser B Phys Biol Sci. 2010; 86(7):731-47. PMC: 3066539. DOI: 10.2183/pjab.86.731. View

Kunz C, Meyer C, Collado M, Geiger L, Garcia-Mantrana I, Bertua-Rios B . Influence of Gestational Age, Secretor, and Lewis Blood Group Status on the Oligosaccharide Content of Human Milk. J Pediatr Gastroenterol Nutr. 2016; 64(5):789-798. DOI: 10.1097/MPG.0000000000001402. View

Kunz C, Rudloff S . Compositional Analysis and Metabolism of Human Milk Oligosaccharides in Infants. Nestle Nutr Inst Workshop Ser. 2017; 88:137-147. DOI: 10.1159/000455398. View

Seppo A, Autran C, Bode L, Jarvinen K . Human milk oligosaccharides and development of cow's milk allergy in infants. J Allergy Clin Immunol. 2016; 139(2):708-711.e5. PMC: 5303147. DOI: 10.1016/j.jaci.2016.08.031. View

Lin A, Autran C, Szyszka A, Escajadillo T, Huang M, Godula K . Human milk oligosaccharides inhibit growth of group B . J Biol Chem. 2017; 292(27):11243-11249. PMC: 5500792. DOI: 10.1074/jbc.M117.789974. View

10.

Thurl S, Henker J, Siegel M, Tovar K, Sawatzki G . Detection of four human milk groups with respect to Lewis blood group dependent oligosaccharides. Glycoconj J. 1998; 14(7):795-9. DOI: 10.1023/a:1018529703106. View

11.

Vandenplas Y, Berger B, Carnielli V, Ksiazyk J, Lagstrom H, Sanchez Luna M . Human Milk Oligosaccharides: 2'-Fucosyllactose (2'-FL) and Lacto-N-Neotetraose (LNnT) in Infant Formula. Nutrients. 2018; 10(9). PMC: 6164445. DOI: 10.3390/nu10091161. View

12.

Mandic Z, Perl Piricki A, Kenjeric D, Hanicar B, Tanasic I . Breast vs. bottle: differences in the growth of Croatian infants. Matern Child Nutr. 2010; 7(4):389-96. PMC: 6860865. DOI: 10.1111/j.1740-8709.2010.00246.x. View

13.

Zivkovic A, German J, Lebrilla C, Mills D . Human milk glycobiome and its impact on the infant gastrointestinal microbiota. Proc Natl Acad Sci U S A. 2010; 108 Suppl 1:4653-8. PMC: 3063602. DOI: 10.1073/pnas.1000083107. View

14.

Dieterich C, Felice J, OSullivan E, Rasmussen K . Breastfeeding and health outcomes for the mother-infant dyad. Pediatr Clin North Am. 2012; 60(1):31-48. PMC: 3508512. DOI: 10.1016/j.pcl.2012.09.010. View

15.

Johnson P, WATKINS W . Purification of the Lewis blood-group gene associated alpha-3/4-fucosyltransferase from human milk: an enzyme transferring fucose primarily to type 1 and lactose-based oligosaccharide chains. Glycoconj J. 1992; 9(5):241-9. DOI: 10.1007/BF00731136. View

16.

Newburg D, Ruiz-Palacios G, Morrow A . Human milk glycans protect infants against enteric pathogens. Annu Rev Nutr. 2005; 25:37-58. DOI: 10.1146/annurev.nutr.25.050304.092553. View

17.

Bai Y, Tao J, Zhou J, Fan Q, Liu M, Hu Y . Fucosylated Human Milk Oligosaccharides and N-Glycans in the Milk of Chinese Mothers Regulate the Gut Microbiome of Their Breast-Fed Infants during Different Lactation Stages. mSystems. 2019; 3(6). PMC: 6306508. DOI: 10.1128/mSystems.00206-18. View

18.

Goehring K, Kennedy A, Prieto P, Buck R . Direct evidence for the presence of human milk oligosaccharides in the circulation of breastfed infants. PLoS One. 2014; 9(7):e101692. PMC: 4085000. DOI: 10.1371/journal.pone.0101692. View

19.

Denborough M, Downing H . Secretor status in asthma and hay fever. J Med Genet. 1968; 5(4):302-5. PMC: 1468666. DOI: 10.1136/jmg.5.4.302. View

20.

Asakuma S, Hatakeyama E, Urashima T, Yoshida E, Katayama T, Yamamoto K . Physiology of consumption of human milk oligosaccharides by infant gut-associated bifidobacteria. J Biol Chem. 2011; 286(40):34583-92. PMC: 3186357. DOI: 10.1074/jbc.M111.248138. View