Genomic Analysis of Shiga Toxin-producing Escherichia Coli O157:H7 from Cattle and Pork-production Related Environments

Overview

Journal NPJ Sci Food

Publisher Springer Nature

Date 2021 Jul 2

PMID 34210979

Citations 6

Authors

Peipei Zhang

Saida Essendoubi

Julia Keenliside

Tim Reuter

Kim Stanford

Robin King

Patricia Lu

Xianqin Yang

Affiliations

Soon will be listed here.

Abstract

Three E. coli O157:H7 outbreaks have been attributed to contaminated pork in Alberta, Canada, recently. This study investigates the phylogenetic relatedness of E. coli O157:H7 from pigs, cattle, and pork-production environments for source attribution. Limited strain diversity was observed using five conventional subtyping methods, with most or all strains being in one subgroup. Whole-genome single nucleotide polymorphism analysis confirmed the recent ancestry of the isolates from all three sources. Most environmental isolates clustered closer with pig isolates than cattle isolates. Also, a direct link was observed between 2018-outbreak environmental isolates and isolates collected from a pig farm in 2018. The majority of pig isolates harbor only one Shiga toxin gene, stx, while 70% (35/50) of the cattle isolates have both stx and stx. The results show some E. coli O157:H7 strains could establish persistence on pig farms and as such, pigs can be a significant source of the organism.

Citing Articles

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