and Counts, Virulence Gene Profile, Antimicrobial Resistance, and Biofilm Formation Capacity During Pig Slaughter Stages

Overview

Journal Life (Basel)

Specialty Biology

Date 2024 Oct 26

PMID 39459561

Authors

Madalena Maria Saldanha Coelho

Emilia Fernanda Agostinho Davanzo

Rebecca Lavarini Dos Santos

Virgilio Hipolito de Lemos Castro

Hayanna Maria Boaventura da Costa

Bruno Stefano Lima Dallago

Simone Perecmanis

Angela Patricia Santana

Affiliations

Soon will be listed here.

Abstract

This study aimed to count and in different locations on pig carcasses (shank, loin, abdomen, shoulder, and jowl) from two slaughterhouses (A and B) between September 2019 and July 2021 during different slaughter stages (after bleeding, after passing through the epilator machine, after manual toileting in the dirty area, before and after evisceration, and after the final washing), as well as verify antimicrobial resistance and biofilm formation capacity. The main points of and contamination were identified in the two slaughterhouses through three collections. The stages with the highest counts were post-bleeding and evisceration in both slaughterhouses and after manual toileting in slaughterhouse B in the first collection. Most isolates were resistant to multiple antimicrobials, with higher resistance frequencies to amoxicillin, ampicillin, chloramphenicol, sulfonamides, and streptomycin. The virulence genes , , and were also detected. Three isolates had all three genes and exhibited resistance to at least six antimicrobial classes (β-lactams, macrolides, aminoglycosides, sulfonamides, amphenicols, and quinolones). isolates also showed a high frequency of strains with moderate and strong in vitro biofilm-forming capacity. This is the first study to characterize microbial contamination by pig slaughter stage in the Federal District region, demonstrating the critical points for hygienic production. was isolated from the surface of pig carcasses, as well as the virulence genes , , and were detected. The multi-antimicrobial resistant isolates also had a moderate-to-strong biofilm formation capacity, thus demonstrating risks to public health.

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