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Isolation of Nuclei from Mouse White Adipose Tissues for Single-nucleus Genomics

Overview
Journal STAR Protoc
Publisher Cell Press
Specialties Biology
Biomedical Engineering
Science
Date 2021 Jun 30
PMID 34189477
Citations 10
Authors
Elvira Laila Van Hauwaert
Ellen Gammelmark
Anitta Kinga Sarvari
Lena Larsen
Ronni Nielsen
Jesper Grud Skat Madsen
Susanne Mandrup
Affiliations
Soon will be listed here.
Abstract

Lipid-filled adipocytes are incompatible with droplet-based single-cell methods, such as 10x Genomics-based technology, thus restricting droplet-based single-cell analyses of adipose tissues to the stromal vascular fraction. To overcome this limitation and obtain cellular and molecular insight into adipose tissue composition and plasticity, single-nucleus sequencing-based technologies can be applied. Here, we provide an optimized protocol for nuclei isolation from mouse adipose tissues suitable for single-nucleus RNA sequencing. This allows for transcriptomic profiling of the entire adipose tissue at single-cell resolution. For complete details on the use of this protocol, please refer to Sárvári et al., 2021.

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