Construction and Characterization of an Multi-Gene Deletion Strain and Evaluation of Its Potential As a Live-Attenuated Vaccine in Grass Carp

Overview

Journal Vaccines (Basel)

Date 2021 Jun 2

PMID 34063680

Citations 8

Authors

Jihong Li

Shilin Ma

Zhi Li

Wei Yu

Peng Zhou

Xiang Ye

Md Sharifull Islam

Yong-An Zhang

Yang Zhou

Jinquan Li

Affiliations

Soon will be listed here.

Abstract

is an important pathogen that causes motile septicemia (MAS) in the aquaculture industry. Aerolysin, hemolysin, serine protease and enterotoxins are considered to be the major virulence factors of . In this study, we constructed a five-gene (A, , , and ) deletion mutant strain (named five-gene deletion strain, AHFGDS) to observe the biological characteristics and detect its potential as a live-attenuated vaccine candidate. AHFGDS displayed highly attenuated and showed increased susceptibility to fish blood and skin mucus killing, while the wild-type strain ZYAH72 was highly virulent. In zebrafish (), AHFGDS showed a 240-fold higher 50% lethal dose (LD50) than that of the wild-type strain. Immunization with AHFGDS by intracelomic injection or immersion routes both provided grass carp () significant protection against the challenge of the strain ZYAH72 or J-1 and protected the fish organs from serious injury. Further agglutinating antibody titer test supported that AHFGDS could elicit a host-adaptive immune response. These results suggested the potential of AHFGDS to serve as a live-attenuated vaccine to control infection in aquaculture.

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