Flow Cytometry-Based Analysis of the Mouse Bone Marrow Stromal and Perivascular Compartment

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2021 May 31

PMID 34057716

Citations 7

Authors

Yuki Matsushita

Wanida Ono

Noriaki Ono

Affiliations

Soon will be listed here.

Abstract

Bone marrow stromal cells (BMSCs) account for an extremely small percentage of total bone marrow cells; therefore, it is technically challenging to harvest a good quantity of BMSCs with good viability using fluorescence-activated cell sorting (FACS). Here, we describe the methods to effectively isolate BMSCs for flow cytometry analyses and subsequent FACS. Use of transgenic reporter lines facilitates FACS-based isolation of BMSCs, aiding to uncover fundamental characteristics of these diverse cell populations.

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