Complement Induces a Transient Increase in Membrane Permeability in Unlysed Erythrocytes

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1988 Aug 1

PMID 3403718

Citations 11

Authors

J A Halperin

A Nicholson-Weller

C Brugnara

D C Tosteson

Affiliations

Soon will be listed here.

Abstract

The effects of low concentrations of human serum on antibody-sensitized sheep erythrocytes (EA) were studied. We report that exposure to low concentrations of serum induced a large but transient increase in the membrane permeability of those EA that do not lyse. This change in the permeability of the erythrocyte membrane resulted in net uptake of Na+ and decrease in cell K+, without affecting the total internal cation content. Although exposure to serum also allowed for net uptake of larger molecules like L-glucose, it did not lead to cell swelling. Experiments with sera genetically deficient in one of the terminal complement components showed that C8, but not C9, was required to produce the observed change in membrane permeability. Therefore, we propose that the C5b-8 complex can mediate the transient increase in permeability observed in unlysed erythrocytes during complement activation by whole serum.

Citing Articles

Inhibition of Complement Retards Ankylosing Spondylitis Progression.

Yang C, Ding P, Wang Q, Zhang L, Zhang X, Zhao J Sci Rep. 2016; 6:34643.

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Role of complement and complement regulatory proteins in the complications of diabetes.

Ghosh P, Sahoo R, Vaidya A, Chorev M, Halperin J Endocr Rev. 2015; 36(3):272-88.

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Activation of endogenously expressed ion channels by active complement in the retinal pigment epithelium.

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Association of the pattern recognition molecule H-ficolin with incident microalbuminuria in an inception cohort of newly diagnosed type 1 diabetic patients: an 18 year follow-up study.

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Evidence for a protective role of the Gardos channel against hemolysis in murine spherocytosis.

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