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A Robust Protocol for the Generation of Human Midbrain Organoids

Overview
Journal STAR Protoc
Publisher Cell Press
Specialties Biology
Biomedical Engineering
Science
Date 2021 May 24
PMID 34027482
Citations 13
Authors
Alise Zagare
Matthieu Gobin
Anna S Monzel
Jens C Schwamborn
Affiliations
Soon will be listed here.
Abstract

The lack of advanced models recapitulating the human brain complexity is still a major obstacle in brain development and neurological disease research. Here, we describe a robust protocol to derive human midbrain organoids from neuroepithelial stem cells. These complex 3D models are characterized by the presence of functional neurons, including dopaminergic neurons and glial cells, making them particularly attractive for the study of Parkinson disease. For complete details on the use and execution of this protocol, please refer to Monzel et al. (2017).

Citing Articles

Cystatin C Secretion in Blood Derivatives and Cellular Models of Idiopathic Parkinson's Disease.

Mommaerts K, Monzel A, Zagare A, Nickels S, Diederich N, Longhino L Parkinsons Dis. 2025; 2025:5149071.

PMID: 39958955 PMC: 11824396. DOI: 10.1155/padi/5149071.

Insulin resistance compromises midbrain organoid neuronal activity and metabolic efficiency predisposing to Parkinson's disease pathology.

Zagare A, Kurlovics J, Almeida C, Ferrante D, Frangenberg D, Vitali A J Tissue Eng. 2025; 16:20417314241295928.

PMID: 39882547 PMC: 11775974. DOI: 10.1177/20417314241295928.

Two- and Three-Dimensional In Vitro Models of Parkinson's and Alzheimer's Diseases: State-of-the-Art and Applications.

Solana-Manrique C, Sanchez-Perez A, Paricio N, Munoz-Descalzo S Int J Mol Sci. 2025; 26(2).

PMID: 39859333 PMC: 11766061. DOI: 10.3390/ijms26020620.

Simple and Cost-Effective Generation of 3D Cell Sheets and Spheroids Using Curvature-Controlled Paraffin Wax Substrates.

Kim H, Koo K, Kim C, Byun M, Park C, Son H Nano Converg. 2024; 11(1):44.

PMID: 39482392 PMC: 11527855. DOI: 10.1186/s40580-024-00451-4.

Unveiling the Neurodegenerative Alterations through Oral Stem Cells.

Tatullo M, Cocco T, Ferretta A, Caroppo R, Marrelli B, Spagnuolo G J Dent Res. 2024; 103(11):1100-1108.

PMID: 39275988 PMC: 11653283. DOI: 10.1177/00220345241265661.

References
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