Design and Application of Mini-libraries of MiRNA Probes for an Efficient and Versatile MiRNA-mRNA Cross-linking

Overview

Journal Chemistry

Specialty Chemistry

Date 2021 May 17

PMID 34000095

Citations 1

Authors

Anna L Malinowska

Artur Laski

Jonathan Hall

Affiliations

Soon will be listed here.

Abstract

MicroRNAs constitute a class of endogenous, non-coding RNAs that influence various processes within the cell. By base-pairing to partially-complementary sites located in the 3' untranslated region of target messenger RNAs, microRNAs participate in post-transcriptional regulation of the majority of human protein-coding genes. Their dysregulation has been related to many pathological processes and diseases. Thus, an in-depth understanding of the microRNA mechanisms of action is crucial. Here, we present a new concept of probe design to achieve an efficient and sequence-independent miRNA-mRNA cross-linking. The new strategy is based on the utilization of a controlled mixture of probes for a chosen miRNA, in which a trioxsalen moiety is introduced at the N -position of a selected cytidine through short oligoethylene glycol-based linkers. In vitro photo-cross-linking experiments with mini-libraries of probes for microRNAs of interest showed variable cross-linking efficiencies, demonstrating a general applicability of the presented approach.

Citing Articles

Design and Application of Mini-libraries of miRNA Probes for an Efficient and Versatile miRNA-mRNA Cross-linking.

Malinowska A, Laski A, Hall J Chemistry. 2021; 27(39):10193-10200.

PMID: 34000095 PMC: 8362200. DOI: 10.1002/chem.202101171.

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