Regulation of Mammalian 3D Genome Organization and Histone H3K9 Dimethylation by H3K9 Methyltransferases

Overview

Journal Commun Biol

Specialty Biology

Date 2021 May 14

PMID 33986449

Citations 14

Authors

Kei Fukuda

Chikako Shimura

Hisashi Miura

Akie Tanigawa

Takehiro Suzuki

Naoshi Dohmae

Ichiro Hiratani

Yoichi Shinkai

Affiliations

Soon will be listed here.

Abstract

Histone H3 lysine 9 dimethylation (H3K9me2) is a highly conserved silencing epigenetic mark. Chromatin marked with H3K9me2 forms large domains in mammalian cells and overlaps well with lamina-associated domains and the B compartment defined by Hi-C. However, the role of H3K9me2 in 3-dimensional (3D) genome organization remains unclear. Here, we investigated genome-wide H3K9me2 distribution, transcriptome, and 3D genome organization in mouse embryonic stem cells following the inhibition or depletion of H3K9 methyltransferases (MTases): G9a, GLP, SETDB1, SUV39H1, and SUV39H2. We show that H3K9me2 is regulated by all five MTases; however, H3K9me2 and transcription in the A and B compartments are regulated by different MTases. H3K9me2 in the A compartments is primarily regulated by G9a/GLP and SETDB1, while H3K9me2 in the B compartments is regulated by all five MTases. Furthermore, decreased H3K9me2 correlates with changes to more active compartmental state that accompanied transcriptional activation. Thus, H3K9me2 contributes to inactive compartment setting.

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