Early Response of Monocyte-derived Macrophages from Vaccinated and Non-vaccinated Goats Against in Vitro Infection with Mycobacterium Avium Subsp. Paratuberculosis

Overview

Journal Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2021 May 13

PMID 33980310

Citations 11

Authors

Noive Arteche-Villasol

Daniel Gutierrez-Exposito

Raquel Vallejo

Jose Espinosa

Natalia Elguezabal

Iraia Ladero-Aunon

Marcos Royo

Maria Del Carmen Ferreras

Julio Benavides

Valentin Perez

Affiliations

Soon will be listed here.

Abstract

Paratuberculosis is a disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). Vaccination is the most cost-effective control method. However, despite the fact that macrophages are the main target cells for this pathogen, the precise mechanisms behind the response of the macrophage to Map infection and how it is modified by vaccination are yet poorly understood. The aim of this study was to investigate the effect of Silirum vaccination in the early immune response of caprine monocyte-derived macrophages (CaMØs). Peripheral blood mononuclear cells (PBMCs) were obtained from vaccinated and non-vaccinated goats, cultured in vitro until differentiation to macrophages and infected with Map. After a 24 h incubation, Map viability and DNA were assessed in culture by viable colony count and real time quantitative polymerase chain reaction (qPCR). In addition, Map phagocytosis and expression of IL-10, IL-12, IFN-γ, TNF-α, IL-17A, IL-1β, iNOS, IL-6 and MIP-1β were also evaluated through immunofluorescence labelling and reverse transcriptase qPCR (RT-qPCR), respectively. A significant reduction of Map viability was observed in both supernatants (P < 0.05) and CaMØs (P < 0.001) from the vaccinated group. Similarly, the percentage of infected CaMØs and the number of internalized Map by CaMØs (P < 0.0001) was higher in the vaccinated group. Finally, iNOS (P < 0.01) and IL-10 were significantly up-regulated in CaMØs from vaccinated goats, whereas only MIP-1β was up-regulated in non-vaccinated animals (P < 0.05). These results show that vaccination modifies the immune response of CaMØs, suggesting that the phagocytosis and microbiocidal activity of macrophages against Map is enhanced after vaccination.

Citing Articles

Evaluation of alternative vaccination routes against paratuberculosis in goats.

Criado M, Silva M, Arteche-Villasol N, Zapico D, Elguezabal N, Molina E Front Vet Sci. 2024; 11:1457849.

PMID: 39664904 PMC: 11631874. DOI: 10.3389/fvets.2024.1457849.

Detection of caprine paratuberculosis (Johne's disease) in pre- and post-vaccinated herds: morphological diagnosis, lesion grading, and bacterial identification.

Stefanova E, Sierra E, Fernandez A, Quesada-Canales O, Paz-Sanchez Y, Colom-Rivero A Front Vet Sci. 2024; 11:1395928.

PMID: 39144076 PMC: 11322454. DOI: 10.3389/fvets.2024.1395928.

No Evidence of Neutrophil Response Modulation in Goats after Immunization against Paratuberculosis with a Heat-Inactivated Vaccine.

Criado M, Silva M, Mendivil P, Molina E, Perez V, Benavides J Animals (Basel). 2024; 14(11).

PMID: 38891741 PMC: 11171245. DOI: 10.3390/ani14111694.

Modeling Paratuberculosis in Laboratory Animals, Cells, or Tissues: A Focus on Their Applications for Pathogenesis, Diagnosis, Vaccines, and Therapy Studies.

Jolly A, Fernandez B, Mundo S, Elguezabal N Animals (Basel). 2023; 13(22).

PMID: 38003170 PMC: 10668694. DOI: 10.3390/ani13223553.

Evaluation of the innate immune response of caprine neutrophils against Mycobacterium avium subspecies paratuberculosis in vitro.

Criado M, Perez V, Arteche-Villasol N, Elguezabal N, Molina E, Benavides J Vet Res. 2023; 54(1):61.

PMID: 37464437 PMC: 10355032. DOI: 10.1186/s13567-023-01193-7.

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