Identification of the C5a Des Arg Cochemotaxin. Homology with Vitamin D-binding Protein (group-specific Component Globulin)

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1988 Jul 1

PMID 3392212

Citations 18

Authors

H D Perez

E Kelly

D Chenoweth

F Elfman

Affiliations

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Abstract

The chemotactic activity of human C5a des Arg is enhanced significantly by an anionic polypeptide (cochemotaxin) in normal human serum and plasma. The cochemotaxin attaches to sialic acid residues within the oligosaccharide chain of native C5a des Arg to form a complex with potent chemotactic activity for human PMN. We investigated the nature of the cochemotaxin and found that vitamin D-binding protein is the putative cochemotaxin. Vitamin D-binding protein enhanced the chemotactic activity of native C5a des Arg, but had no effect on the chemotactic activity of either native C5a or FMLP. Sialic acid prevented both enhancement by vitamin D-binding protein of the chemotactic activity of native C5a des Arg and formation of C5a des Arg-vitamin D-binding protein complexes, detected by molecular sieve chromatography. Furthermore, vitamin D-binding protein and cochemotaxin exhibited identical molecular weights, isoelectric points, antigenic reactivity, and amino acid composition.

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References

Bokisch V, MULLER-EBERHARD H . Anaphylatoxin inactivator of human plasma: its isolation and characterization as a carboxypeptidase. J Clin Invest. 1970; 49(12):2427-36. PMC: 322744. DOI: 10.1172/JCI106462. View

Zigmond S, Hirsch J . Leukocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor. J Exp Med. 1973; 137(2):387-410. PMC: 2139498. DOI: 10.1084/jem.137.2.387. View

Ofarrell P . High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975; 250(10):4007-21. PMC: 2874754. View

Keller H, Hess M, COTTIER H . The chemokinetic effect of serum albumin. Experientia. 1977; 33(10):1386-7. DOI: 10.1007/BF01920196. View

Fernandez H, Henson P, Otani A, Hugli T . Chemotactic response to human C3a and C5a anaphylatoxins. I. Evaluation of C3a and C5a leukotaxis in vitro and under stimulated in vivo conditions. J Immunol. 1978; 120(1):109-15. View

Perez H, Lipton M, Goldstein I . A specific inhibitor of complement (C5)-derived chemotactic activity in serum from patients with systemic lupus erythematosus. J Clin Invest. 1978; 62(1):29-38. PMC: 371733. DOI: 10.1172/JCI109110. View

Perez H, Chenoweth D, Goldstein I . Attachment of human C5a des Arg to its cochemotaxin is required for maximum expression of chemotactic activity. J Clin Invest. 1986; 78(6):1589-95. PMC: 423925. DOI: 10.1172/JCI112751. View

Webster R, Hong S, JOHNSTON Jr R, Henson P . Biologial effects of the human complement fragments C5a and C5ades Arg on neutrophil function. Immunopharmacology. 1980; 2(3):201-19. DOI: 10.1016/0162-3109(80)90050-8. View

Perez H, Goldstein I, Webster R, Henson P . Enhancement of the chemotactic activity of human C5a des Arg by an anionic polypeptide ("cochemotaxin") in normal serum and plasma. J Immunol. 1981; 126(2):800-4. View

10.

Gerard C, Chenoweth D, Hugli T . Response of human neutrophils to C5a: a role for the oligosaccharide moiety of human C5ades Arg-74 but not of C5a in biologic activity. J Immunol. 1981; 127(5):1978-82. View

11.

Perez H, Elfman F, Lobo E, Sklar L, Chenoweth D, Hooper C . A derivative of wheat germ agglutinin specifically inhibits formyl-peptide-induced polymorphonuclear leukocyte chemotaxis by blocking re-expression (or recycling) of receptors. J Immunol. 1986; 136(5):1803-12. View

12.

Perez H, Elfman F, Chenoweth D, Hooper C . Preparation and characterization of a derivative of wheat germ agglutinin which specifically inhibits polymorphonuclear leukocyte chemotaxis to the synthetic chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine. J Immunol. 1986; 136(5):1813-9. View

13.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View