Feeding Pattern, Biochemical, Anthropometric and Histological Effects of Prolonged Access to Sucrose, Honey and Glucose-fructose Solutions in Wistar Rats

Overview

Journal Nutr Res Pract

Specialty Nutritional Sciences

Date 2021 Apr 12

PMID 33841723

Citations 1

Authors

Carmen Alejandrina Virgen-Carrillo

Alma Gabriela Martinez Moreno

Juan Jose Rodriguez-Gudino

Jessica Elizabeth Pineda-Lozano

Affiliations

Soon will be listed here.

Abstract

Background/objectives: The exposure to sucrose in rats has mimic abnormalities attributed to metabolic syndrome (MetS). The effects of honey bee and "free" glucose and fructose, have not been explored in this context. The aim was to expose Wistar rodents to sucrose solution (SS), honey solution (HS) and fructose/glucose solution (GFS) at 30% to assess their effects.

Subjects/methods: HS (n = 10), SS (n = 10) and GFS (n = 10) groups were formed. Solutions were along 14-weeks.

Results: Between solutions consumptions, honey was significantly 42% higher ( = 0.000), while similar consumption was observed among GFS and SS. The feeding pattern of HS consumption was irregular along experiment; while the food intake pattern showed the similar trend among groups along time. Non statistical differences were obtained in any biochemical and anthropometric measure, however, a higher concentration of leptin (721 ± 507 pg/mL), lower concentration of total cholesterol (TC; 48.87 ± 2.41 mg/100 mL), very low density lipoprotein (VLDL; 16.47 ± 6.55 mg/100 mL) and triglycerides (82.37 ± 32.77 mg/100 mL) was obtained in SS group. For anthropometric values, HS showed less total adipose tissue (AT; average 26 vs. 31-33 g) and adiposity index (average 6.11 vs. 7.6). Due to sugar-sweetened beverages consumption increases the risk for the development of chronic diseases; correlations between fluid intake and anthropometric and biochemical parameters were assessed. A moderate correlation was obtained in groups with the weight of total AT and solution intake; for the weight gain in GFS group and for triglycerides in HS and GFS. The highest hepatic tissue damage was observed in SS group with multiple intracytoplasmic vacuoles, atypia changes, moderate pleomorphism and hepatocellular necrosis.

Conclusions: In spite of the significantly higher consumption of HS, biochemical, anthropometrical and histological effects were not remarkably different in comparision to other sweeteners.

Citing Articles

Relationship between Prenatal or Postnatal Exposure to Pesticides and Obesity: A Systematic Review.

Pinos H, Carrillo B, Merchan A, Biosca-Brull J, Perez-Fernandez C, Colomina M Int J Environ Res Public Health. 2021; 18(13).

PMID: 34281107 PMC: 8295932. DOI: 10.3390/ijerph18137170.

References

Luo S, Monterosso J, Sarpelleh K, Page K . Differential effects of fructose versus glucose on brain and appetitive responses to food cues and decisions for food rewards. Proc Natl Acad Sci U S A. 2015; 112(20):6509-14. PMC: 4443321. DOI: 10.1073/pnas.1503358112. View

Erejuwa O, Sulaiman S, Ab Wahab M . Oligosaccharides might contribute to the antidiabetic effect of honey: a review of the literature. Molecules. 2011; 17(1):248-66. PMC: 6268503. DOI: 10.3390/molecules17010248. View

Kendig M, Boakes R, Rooney K, Corbit L . Chronic restricted access to 10% sucrose solution in adolescent and young adult rats impairs spatial memory and alters sensitivity to outcome devaluation. Physiol Behav. 2013; 120:164-72. DOI: 10.1016/j.physbeh.2013.08.012. View

Sanchez-Lozada L, Mu W, Roncal C, Sautin Y, Abdelmalek M, Reungjui S . Comparison of free fructose and glucose to sucrose in the ability to cause fatty liver. Eur J Nutr. 2009; 49(1):1-9. PMC: 2805058. DOI: 10.1007/s00394-009-0042-x. View

da Silva P, Gauche C, Gonzaga L, Costa A, Fett R . Honey: Chemical composition, stability and authenticity. Food Chem. 2015; 196:309-23. DOI: 10.1016/j.foodchem.2015.09.051. View

Volkow N, Wise R . How can drug addiction help us understand obesity?. Nat Neurosci. 2005; 8(5):555-60. DOI: 10.1038/nn1452. View

Chepulis L, Starkey N, Waas J, Molan P . The effects of long-term honey, sucrose or sugar-free diets on memory and anxiety in rats. Physiol Behav. 2009; 97(3-4):359-68. DOI: 10.1016/j.physbeh.2009.03.001. View

Erejuwa O, Sulaiman S, Ab Wahab M . Fructose might contribute to the hypoglycemic effect of honey. Molecules. 2012; 17(2):1900-15. PMC: 6268125. DOI: 10.3390/molecules17021900. View

Fernstrom J, Munger S, Sclafani A, de Araujo I, Roberts A, Molinary S . Mechanisms for sweetness. J Nutr. 2012; 142(6):1134S-41S. PMC: 3738222. DOI: 10.3945/jn.111.149567. View

10.

Ramli N, Chin K, Zarkasi K, Ahmad F . A Review on the Protective Effects of Honey against Metabolic Syndrome. Nutrients. 2018; 10(8). PMC: 6115915. DOI: 10.3390/nu10081009. View

11.

Erejuwa O, Sulaiman S, Ab Wahab M . Honey--a novel antidiabetic agent. Int J Biol Sci. 2012; 8(6):913-34. PMC: 3399220. DOI: 10.7150/ijbs.3697. View

12.

Ajibola A, Chamunorwa J, Erlwanger K . Comparative effect of cane syrup and natural honey on abdominal viscera of growing male and female rats. Indian J Exp Biol. 2013; 51(4):303-12. View

13.

Chepulis L, Starkey N . The long-term effects of feeding honey compared with sucrose and a sugar-free diet on weight gain, lipid profiles, and DEXA measurements in rats. J Food Sci. 2008; 73(1):H1-7. DOI: 10.1111/j.1750-3841.2007.00592.x. View

14.

Popkin B . Patterns of beverage use across the lifecycle. Physiol Behav. 2010; 100(1):4-9. PMC: 2849916. DOI: 10.1016/j.physbeh.2009.12.022. View

15.

Kenny P . Reward mechanisms in obesity: new insights and future directions. Neuron. 2011; 69(4):664-79. PMC: 3057652. DOI: 10.1016/j.neuron.2011.02.016. View

16.

Malik V, Hu F . Sugar-Sweetened Beverages and Cardiometabolic Health: An Update of the Evidence. Nutrients. 2019; 11(8). PMC: 6723421. DOI: 10.3390/nu11081840. View

17.

Saklayen M . The Global Epidemic of the Metabolic Syndrome. Curr Hypertens Rep. 2018; 20(2):12. PMC: 5866840. DOI: 10.1007/s11906-018-0812-z. View

18.

Ackroff K, Touzani K, Peets T, Sclafani A . Flavor preferences conditioned by intragastric fructose and glucose: differences in reinforcement potency. Physiol Behav. 2001; 72(5):691-703. DOI: 10.1016/s0031-9384(01)00442-5. View

19.

Atkinson F, Foster-Powell K, Brand-Miller J . International tables of glycemic index and glycemic load values: 2008. Diabetes Care. 2008; 31(12):2281-3. PMC: 2584181. DOI: 10.2337/dc08-1239. View

20.

Marriott B, Olsho L, Hadden L, Connor P . Intake of added sugars and selected nutrients in the United States, National Health and Nutrition Examination Survey (NHANES) 2003-2006. Crit Rev Food Sci Nutr. 2010; 50(3):228-58. DOI: 10.1080/10408391003626223. View