Developments in Solution-state NMR Yield Broader and Deeper Views of the Dynamic Ensembles of Nucleic Acids

Overview

Journal Curr Opin Struct Biol

Date 2021 Apr 9

PMID 33836446

Citations 26

Authors

Bei Liu

Honglue Shi

Hashim M Al-Hashimi

Affiliations

Soon will be listed here.

Abstract

Nucleic acids do not fold into a single conformation, and dynamic ensembles are needed to describe their propensities to cycle between different conformations when performing cellular functions. We review recent advances in solution-state nuclear magnetic resonance (NMR) methods and their integration with computational techniques that are improving the ability to probe the dynamic ensembles of DNA and RNA. These include computational approaches for predicting chemical shifts from structure and generating conformational libraries from sequence, measurements of exact nuclear Overhauser effects, development of new probes to study chemical exchange using relaxation dispersion, faster and more sensitive real-time NMR techniques, and new NMR approaches to tackle large nucleic acid assemblies. We discuss how these advances are leading to new mechanistic insights into gene expression and regulation.

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