Directed Evolution in Mammalian Cells

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2021 Apr 8

PMID 33828274

Citations 33

Authors

Samuel J Hendel

Matthew D Shoulders

Affiliations

Soon will be listed here.

Abstract

Directed evolution experiments are typically carried out using in vitro systems, bacteria, or yeast-even when the goal is to probe or modulate mammalian biology. Performing directed evolution in systems that do not match the intended mammalian environment severely constrains the scope and functionality of the targets that can be evolved. We review new platforms that are now making it possible to use the mammalian cell itself as the setting for directed evolution and present an overview of frontier challenges and high-impact targets for this approach.

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