Ropivacaine Inhibits Proliferation, Migration, and Invasion While Inducing Apoptosis of Glioma Cells by Regulating the SNHG16/miR-424-5p Axis

Overview

Journal Open Life Sci

Specialty Biology

Date 2021 Apr 5

PMID 33817285

Citations 6

Authors

Rong Liu

Min Wu

Guiju Xu

Lu Ju

Jinhui Xiao

Wei Zhong

Xiao He

Yan Yang

Affiliations

Soon will be listed here.

Abstract

Background: Regional anesthesia has anti-proliferative and pro-apoptotic effects in various cancers. Therefore, the purpose of this study was to investigate the effects of ropivacaine on the proliferation, migration, invasion, and apoptosis of glioma cells .

Methods: Under ropivacaine stimulation conditions, proliferation, apoptosis, migration, and invasion of glioma cells were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2-tetrazol-3-ium bromide (MTT), flow cytometry, and transwell assays, respectively. Western blot assay was employed to measure the protein expression levels in glioma cells. The expression levels of small nucleolar RNA host gene 16 (SNHG16) and miR-424-5p were assessed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The interaction relationship between SNHG16 and miR-424-5p was predicted and confirmed using a bioinformatics database and dual-luciferase reporter, RNA immunoprecipitation (RIP) and RNA pull-down assays.

Results: After treatment with ropivacaine, proliferation, migration, and invasion were repressed while apoptosis was enhanced in glioma cells in a dose-depended manner. In addition, ropivacaine impeded SNHG16 expression in glioma cells. Importantly, overexpression of SNHG16 abolished the ropivacaine-induced effects on glioma cells. Analogously, knockdown of miR-424-5p counteracted the function of ropivacaine in glioma cells. We also found that SNHG16 bound to miR-424-5p and negatively regulated miR-424-5p expression in glioma cells. The rescue experiments indicated that ropivacaine might regulate glioma progression by targeting the SNHG16/miR-424-5p axis.

Conclusion: Our findings revealed the anti-tumor effects of ropivacaine in glioma by targeting the SNHG16/miR-424-5p axis. These data might extend the understanding of regulatory mechanisms by which ropivacaine could suppress glioma development.

Citing Articles

Trial watch: local anesthetics in cancer therapy.

Carnet Le Provost K, Kepp O, Kroemer G, Bezu L Oncoimmunology. 2024; 13(1):2308940.

PMID: 38504848 PMC: 10950281. DOI: 10.1080/2162402X.2024.2308940.

Ropivacaine suppresses the progression of renal cell carcinoma through regulating the lncRNA RMRP/EZH2/CCDC65 axis.

Xiong Y, Zheng X, Deng H Daru. 2023; 32(1):121-132.

PMID: 38008820 PMC: 11087436. DOI: 10.1007/s40199-023-00492-w.

Role of miR-424 in the carcinogenesis.

Ghafouri-Fard S, Askari A, Hussen B, Taheri M, Dilmaghani N Clin Transl Oncol. 2023; 26(1):16-38.

PMID: 37178445 PMC: 10761534. DOI: 10.1007/s12094-023-03209-2.

Impact of local anesthetics on epigenetics in cancer.

Bezu L, Kepp O, Kroemer G Front Oncol. 2022; 12:849895.

PMID: 36110954 PMC: 9468863. DOI: 10.3389/fonc.2022.849895.

MicroRNA-424-5p Alleviates Isoflurane Anesthesia-Induced Neurotoxicity in Human Embryonic Stem Cell-Derived Neurons by Targeting FASN.

Gu X, Yue W, Xiu M, Zhang Q, Xie R Comput Math Methods Med. 2022; 2022:2517463.

PMID: 35872948 PMC: 9300301. DOI: 10.1155/2022/2517463.

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