MicroRNA-424-5p Alleviates Isoflurane Anesthesia-Induced Neurotoxicity in Human Embryonic Stem Cell-Derived Neurons by Targeting FASN

Overview

Journal Comput Math Methods Med

Publisher Hindawi

Specialty Medical Informatics

Date 2022 Jul 25

PMID 35872948

Authors

Xiaojiao Gu

Wei Yue

Mingyu Xiu

Quanyun Zhang

Rufeng Xie

Affiliations

Soon will be listed here.

Abstract

Isoflurane (ISO) is a type of anesthetic that might cause neurotoxicity in children. Although miR-424-5p is considerably downregulated in ISO-treated rat brain samples, its physiological role in ISO-induced neuronal injury in human embryonic stem cell-derived neurons remains unknown (hESC-derived neurons). miR-424-5p expression and fatty acid synthase (FASN) in ISO-treated hESC-derived neurons were tested via qRT-PCR. The amount of protein for Bax, Cleaved-caspase-8, Bcl-2, and FASN was investigated through western blot analysis. The viability and apoptosis of hESC-derived neurons were estimated through cell counting kit-8 assessment and TUNEL assay, accordingly. Superoxide dismutase, glutathione, and malondialdehyde levels were discovered via corresponding kits. The contents of inflammatory factors including interleukin-6 and tumor necrosis factor- were examined by enzyme-linked immunosorbent assays. The combination between FASN and miR-424-5p was resolute via dual-luciferase reporter assessment. After exposure to ISO, induced neurotoxicity and a decreased miR-424-5p production were identified in hESC-derived neurons. Upregulation of miR-424-5p repressed ISO-induced apoptosis and mitigated ISO-induced inflammatory response and oxidative stress in vitro. FASN expression levels were reduced by elevation of miR-424-5p and upregulated after ISO treatment. Mechanically, FASN was directly targeted by miR-424-5p in hESC-derived neurons. Of note, the miR-424-5p elevation-suppressed neuronal apoptosis, inflammatory response, and oxidative stress were countered by upregulation of FASN.

Citing Articles

Retracted: MicroRNA-424-5p Alleviates Isoflurane Anesthesia-Induced Neurotoxicity in Human Embryonic Stem Cell-Derived Neurons by Targeting FASN.

Methods In Medicine C Comput Math Methods Med. 2023; 2023:9851824.

PMID: 37538451 PMC: 10396615. DOI: 10.1155/2023/9851824.

MicroRNAs-Based Theranostics against Anesthetic-Induced Neurotoxicity.

Minz R, Sharma P, Negi A, Kesari K Pharmaceutics. 2023; 15(7).

PMID: 37514018 PMC: 10385075. DOI: 10.3390/pharmaceutics15071833.

References

Zhang C, Lu J, Su H, Yang J, Zhou D . Fatty acid synthase cooperates with protrudin to facilitate membrane outgrowth of cellular protrusions. Sci Rep. 2017; 7:46569. PMC: 5399442. DOI: 10.1038/srep46569. View

Zhang R, Chen L, Huang F, Wang X, Li C . Long non-coding RNA NEAT1 promotes lipopolysaccharide-induced acute lung injury by regulating miR-424-5p/MAPK14 axis. Genes Genomics. 2021; 43(7):815-827. DOI: 10.1007/s13258-021-01103-1. View

Zhao X, Yang Z, Liang G, Wu Z, Peng Y, Joseph D . Dual effects of isoflurane on proliferation, differentiation, and survival in human neuroprogenitor cells. Anesthesiology. 2013; 118(3):537-49. PMC: 3580019. DOI: 10.1097/ALN.0b013e3182833fae. View

Chen H, He Y, Chen S, Qi S, Shen J . Therapeutic targets of oxidative/nitrosative stress and neuroinflammation in ischemic stroke: Applications for natural product efficacy with omics and systemic biology. Pharmacol Res. 2020; 158:104877. DOI: 10.1016/j.phrs.2020.104877. View

Zhang Y, Liu J, Xie C, Wu P . Overexpression of miR-133b protects against isoflurane-induced learning and memory impairment. Exp Ther Med. 2021; 22(5):1207. PMC: 8422403. DOI: 10.3892/etm.2021.10641. View

Evered L, Scott D, Silbert B . Cognitive decline associated with anesthesia and surgery in the elderly: does this contribute to dementia prevalence?. Curr Opin Psychiatry. 2017; 30(3):220-226. DOI: 10.1097/YCO.0000000000000321. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Wang Q, Ge X, Zhang J, Chen L . Effect of lncRNA WT1-AS regulating WT1 on oxidative stress injury and apoptosis of neurons in Alzheimer's disease via inhibition of the miR-375/SIX4 axis. Aging (Albany NY). 2020; 12(23):23974-23995. PMC: 7762490. DOI: 10.18632/aging.104079. View

Wang X, Liu D, Huang H, Wang Z, Hou T, Yang X . A Novel MicroRNA-124/PTPN1 Signal Pathway Mediates Synaptic and Memory Deficits in Alzheimer's Disease. Biol Psychiatry. 2017; 83(5):395-405. DOI: 10.1016/j.biopsych.2017.07.023. View

10.

Ni Mhuireachtaigh R, OGorman D . Anesthesia in pregnant patients for nonobstetric surgery. J Clin Anesth. 2006; 18(1):60-6. DOI: 10.1016/j.jclinane.2004.11.009. View

11.

Dong Y, Hong W, Tang Z, Gao Y, Wu X, Liu H . Sevoflurane leads to learning and memory dysfunction via breaking the balance of tPA/PAI-1. Neurochem Int. 2020; 139:104789. DOI: 10.1016/j.neuint.2020.104789. View

12.

Xiang Y, Zhang Y, Xia Y, Zhao H, Liu A, Chen Y . LncRNA MEG3 targeting miR-424-5p via MAPK signaling pathway mediates neuronal apoptosis in ischemic stroke. Aging (Albany NY). 2020; 12(4):3156-3174. PMC: 7066902. DOI: 10.18632/aging.102790. View

13.

Chen D, Hu S, Wu Z, Liu J, Li S . The Role of MiR-132 in Regulating Neural Stem Cell Proliferation, Differentiation and Neuronal Maturation. Cell Physiol Biochem. 2018; 47(6):2319-2330. DOI: 10.1159/000491543. View

14.

Jiang L, Yang F, Zhao Q, Pan L . MicroRNA-665 mediates propofol-induced cell apoptosis in human stem cell-derived neurons. Bioengineered. 2019; 10(1):493-500. PMC: 6844390. DOI: 10.1080/21655979.2019.1682105. View

15.

Itskovitz-Eldor J, Schuldiner M, Karsenti D, Eden A, Yanuka O, Amit M . Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Mol Med. 2000; 6(2):88-95. PMC: 1949933. View

16.

Wang M, Liu H, Xu L, Li M, Zhao M . The Protective Effect of Notoginsenoside R1 on Isoflurane-Induced Neurological Impairment in the Rats via Regulating miR-29a Expression and Neuroinflammation. Neuroimmunomodulation. 2021; 29(1):70-76. DOI: 10.1159/000518215. View

17.

Zhao Z, Ma L, Li Y, Zhang Q, Wang Y, Tai Y . MiR-124 protects against cognitive dysfunction induced by sevoflurane anesthesia in vivo and in vitro through targeting calpain small subunit 1 via NF-κB signaling pathway. Adv Clin Exp Med. 2021; 30(7):701-709. DOI: 10.17219/acem/134740. View

18.

Wang L, Zheng M, Wu S, Niu Z . MicroRNA-188-3p is involved in sevoflurane anesthesia-induced neuroapoptosis by targeting MDM2. Mol Med Rep. 2018; 17(3):4229-4236. PMC: 5802194. DOI: 10.3892/mmr.2018.8437. View

19.

Rha E, Kim J, Yoo G . Volume Measurement of Various Tissues Using the Image J Software. J Craniofac Surg. 2015; 26(6):e505-6. DOI: 10.1097/SCS.0000000000002022. View

20.

Chen Y, Zhou Z, Wang Y . Prediction and analysis of weighted genes in isoflurane induced general anesthesia based on network analysis. Int J Neurosci. 2019; 130(6):610-620. DOI: 10.1080/00207454.2019.1701452. View