Seroprevalence and Associated Risk Factors for Vector-borne Pathogens in Dogs from Egypt

Overview

Journal Parasit Vectors

Publisher Biomed Central

Specialties Parasitology
Tropical Medicine

Date 2021 Mar 23

PMID 33752744

Citations 26

Authors

Abdelfattah Selim

Abdullah D Alanazi

Alireza Sazmand

Domenico Otranto

Affiliations

Soon will be listed here.

Abstract

Background: Dogs play an important role as reservoirs of many zoonotic vector-borne pathogens worldwide, yet reports of canine vector-borne diseases (CVBDs) in Egypt are scarce.

Methods: Serum samples were collected from pet dogs (n = 500) of the three most common breeds (German Shepherd, Rottweiler and Pit Bull) in five Governates of Cairo (n = 230), Giza (n = 110), Al-Qalyubia (n = 60), Al-Gharbia (n = 60) and Kafr El-Sheikh (n = 40) with a hot desert climate. The presence of antibodies to Anaplasma spp. (A. phagocytophilum, A. platys), Ehrlichia spp. (E. canis, E. chaffeensis, E. ewingii), Borrelia burgdorferi (s.l.) and Dirofilaria immitis were assessed using IDEXX SNAP 4Dx ELISA tests. For each pathogen, risk factors (i.e. geographical area, keeping condition, sex, age, breed, tick infestation, weekly sanitation of dog enclosures and application of ectoparasiticides) were evaluated by logistic regression approach.

Results: In total, 18.2% (n = 91, 95% CI 15.1-21.8) of dogs scored seropositive for at least one pathogen, the most frequent being Ehrlichia spp. (n = 56; 11.2%; 95% CI 8.7-14.3) followed by Anaplasma spp. (n = 33; 6.6%, 95% CI 4.7-9.1), Borrelia burgdorferi (s.l.) (n = 9; 1.8%, 95% CI 0.9-3.4) and D. immitis (n = 7; 1.4%, 95% CI 0.9-2.9). In the tested population, 15.4% (95% CI 12.5-18.8) of dogs were exposed to a single pathogen while 2.4 (95% CI 1.4-4.2) and 0.4% (95% CI 0.1-1.4) were simultaneously exposed to two or three pathogens, respectively. Major risk factors associated with VBDs were living outdoors (Anaplasma spp., P = 0.0001; Ehrlichia spp., P = 0.0001), female sex (Ehrlichia spp., P = 0.005), German Shepherd breed (Anaplasma spp., P = 0.04; Ehrlichia spp., P = 0.03), tick infestation (Anaplasma spp., P = 0.0001; Ehrlichia spp., P = 0.0001; B. burgdorferi (s.l.), P = 0.003; D. immitis, P = 0.02), irregular sanitation (Anaplasma spp., P = 0.0001; Ehrlichia spp., P = 0.0001; B. burgdorferi (s.l.), P = 0.002; D. immitis, P = 0.01) and not using ectoparasiticides (Anaplasma spp., P = 0.0001; Ehrlichia spp., P = 0.0001; B. burgdorferi (s.l.), P = 0.007).

Conclusion: To our knowledge, this is the first large-scale seroepidemiological study of CVBDs in Egypt. Considering that all of the detected pathogens are potentially zoonotic, effective ectoparasite control strategies, regular examination of pet dogs and successful chemoprophylaxis are advocated.

Citing Articles

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References

Yuasa Y, Hsu T, Chou C, Huang C, Huang W, Chang C . The comparison of spatial variation and risk factors between mosquito-borne and tick-borne diseases: Seroepidemiology of Ehrlichia canis, Anaplasma species, and Dirofilaria immitis in dogs. Comp Immunol Microbiol Infect Dis. 2012; 35(6):599-606. DOI: 10.1016/j.cimid.2012.08.001. View

de la Fuente J, Antunes S, Bonnet S, Cabezas-Cruz A, Domingos A, Estrada-Pena A . Tick-Pathogen Interactions and Vector Competence: Identification of Molecular Drivers for Tick-Borne Diseases. Front Cell Infect Microbiol. 2017; 7:114. PMC: 5383669. DOI: 10.3389/fcimb.2017.00114. View

Dantas-Torres F, Chomel B, Otranto D . Ticks and tick-borne diseases: a One Health perspective. Trends Parasitol. 2012; 28(10):437-46. DOI: 10.1016/j.pt.2012.07.003. View

Otranto D, Dantas-Torres F, Breitschwerdt E . Managing canine vector-borne diseases of zoonotic concern: part one. Trends Parasitol. 2009; 25(4):157-63. DOI: 10.1016/j.pt.2009.01.003. View

Angelou A, Gelasakis A, Verde N, Pantchev N, Schaper R, Chandrashekar R . Prevalence and risk factors for selected canine vector-borne diseases in Greece. Parasit Vectors. 2019; 12(1):283. PMC: 6547445. DOI: 10.1186/s13071-019-3543-3. View

Nguyen V, Colella V, Greco G, Fang F, Nurcahyo W, Hadi U . Molecular detection of pathogens in ticks and fleas collected from companion dogs and cats in East and Southeast Asia. Parasit Vectors. 2020; 13(1):420. PMC: 7429691. DOI: 10.1186/s13071-020-04288-8. View

Kilpatrick A, Randolph S . Drivers, dynamics, and control of emerging vector-borne zoonotic diseases. Lancet. 2012; 380(9857):1946-55. PMC: 3739480. DOI: 10.1016/S0140-6736(12)61151-9. View

Breitschwerdt E, Hegarty B, Hancock S . Sequential evaluation of dogs naturally infected with Ehrlichia canis, Ehrlichia chaffeensis, Ehrlichia equi, Ehrlichia ewingii, or Bartonella vinsonii. J Clin Microbiol. 1998; 36(9):2645-51. PMC: 105178. DOI: 10.1128/JCM.36.9.2645-2651.1998. View

Ismail N, Bloch K, McBride J . Human ehrlichiosis and anaplasmosis. Clin Lab Med. 2010; 30(1):261-92. PMC: 2882064. DOI: 10.1016/j.cll.2009.10.004. View

10.

Cardoso L, Mendao C, de Carvalho L . Prevalence of Dirofilaria immitis, Ehrlichia canis, Borrelia burgdorferi sensu lato, Anaplasma spp. and Leishmania infantum in apparently healthy and CVBD-suspect dogs in Portugal--a national serological study. Parasit Vectors. 2012; 5:62. PMC: 3353209. DOI: 10.1186/1756-3305-5-62. View

11.

Lee S, Lee H, Park J, Yoon S, Seo H, Noh J . Prevalence of antibodies against Anaplasma spp., Borrelia burgdorferi sensu lato, Babesia gibsoni, and Ehrlichia spp. in dogs in the Republic of Korea. Ticks Tick Borne Dis. 2020; 11(4):101412. DOI: 10.1016/j.ttbdis.2020.101412. View

12.

Sainz A, Roura X, Miro G, Estrada-Pena A, Kohn B, Harrus S . Guideline for veterinary practitioners on canine ehrlichiosis and anaplasmosis in Europe. Parasit Vectors. 2015; 8:75. PMC: 4324656. DOI: 10.1186/s13071-015-0649-0. View

13.

Harrus S, Aroch I, Lavy E, Bark H . Clinical manifestations of infectious canine cyclic thrombocytopenia. Vet Rec. 1997; 141(10):247-50. DOI: 10.1136/vr.141.10.247. View

14.

Mendoza-Roldan J, Manoj R, Latrofa M, Iatta R, Annoscia G, Lovreglio P . Role of reptiles and associated arthropods in the epidemiology of rickettsioses: A one health paradigm. PLoS Negl Trop Dis. 2021; 15(2):e0009090. PMC: 7888606. DOI: 10.1371/journal.pntd.0009090. View

15.

Sazmand A, Harl J, Eigner B, Hodzic A, Beck R, Hekmatimoghaddam S . Vector-borne bacteria in blood of camels in Iran: New data and literature review. Comp Immunol Microbiol Infect Dis. 2019; 65:48-53. PMC: 7112553. DOI: 10.1016/j.cimid.2019.04.004. View

16.

Alanazi A, Nguyen V, Alyousif M, Manoj R, Alouffi A, Donato R . Ticks and associated pathogens in camels (Camelus dromedarius) from Riyadh Province, Saudi Arabia. Parasit Vectors. 2020; 13(1):110. PMC: 7048137. DOI: 10.1186/s13071-020-3973-y. View

17.

Stuen S, Granquist E, Silaghi C . Anaplasma phagocytophilum--a widespread multi-host pathogen with highly adaptive strategies. Front Cell Infect Microbiol. 2013; 3:31. PMC: 3717505. DOI: 10.3389/fcimb.2013.00031. View

18.

Panarese R, Iatta R, Lia R, Passantino G, Ciccarelli S, Gernone F . Dirofilarioses in two cats in southern Italy. Parasitol Res. 2021; 120(12):4247-4251. DOI: 10.1007/s00436-021-07127-6. View

19.

Otranto D, Iatta R, Baneth G, Cavalera M, Bianco A, Parisi A . High prevalence of vector-borne pathogens in domestic and wild carnivores in Iraq. Acta Trop. 2019; 197:105058. DOI: 10.1016/j.actatropica.2019.105058. View

20.

Breitschwerdt E, Hegarty B, Qurollo B, Saito T, Maggi R, Blanton L . Intravascular persistence of Anaplasma platys, Ehrlichia chaffeensis, and Ehrlichia ewingii DNA in the blood of a dog and two family members. Parasit Vectors. 2014; 7:298. PMC: 4089936. DOI: 10.1186/1756-3305-7-298. View