» Articles » PMID: 33751359

Trelagliptin Alleviates Lipopolysaccharide (LPS)-Induced Inflammation and Oxidative Stress in Acute Lung Injury Mice

Overview
Journal Inflammation
Date 2021 Mar 22
PMID 33751359
Citations 25
Authors
Affiliations
Soon will be listed here.
Abstract

Acute lung injury (ALI) is an urgent disease lacking effective therapies, resulting in relatively high morbidity and mortality. The pathological mechanism of ALI is reported to be related to excessive inflammation and activated oxidative stress. The present study aims to investigate the protective effects of the DPP-4 inhibitor Trelagliptin against lipopolysaccharide (LPS)-induced ALI and the underlying mechanism. LPS was used to induce ALI mice models. The pathological condition of ALI mice was evaluated using MPO activity assay, lung wet to dry weight ratio detection, and HE staining on the lung tissues. Lung function was assessed using a spirometer. The oxidative stress level in the lung tissues was checked by MDA measurement and GPx detection using commercial kits. The leukocyte and neutrophil numbers were determined using a hemocytometer and the total concentration of protein in the BALF was detected using a bicinchoninic acid method. The expression levels of TNF-α, IL-6, and CXCL2 in the lung tissues were evaluated using qRT-PCR and ELISA. Western blot analysis was used to determine the expression levels of TLR4 and p-NF-κB p65. LPS-induced elevated MPO activity, pulmonary wet to dry weight ratio, airway resistance (RAW), the total number of leukocytes and neutrophils, production of inflammatory factors, decreased pulmonary dynamic compliance (Cdyn), and peak expiratory flow (PEF), and an aggravated histopathological state (such as disordered alveolar structure, significant pulmonary interstitial edema, and large numbers of red blood cells and inflammatory cells in the alveolar cavity) were significantly reversed by the administration of Trelagliptin. The TLR4/NF-κB signaling pathway was activated and oxidative stress was induced by stimulation with LPS; however, both effects were suppressed by the administration of Trelagliptin. Trelagliptin might alleviate LPS-induced inflammation and oxidative stress in acute lung injury mice.

Citing Articles

Extracecellulr vesicles (EVs) microRNAs (miRNAs) derived from mesenchymal stem cells (MSCs) in osteoarthritis (OA); detailed role in pathogenesis and possible therapeutics.

Pakdaman Kolour S, Nematollahi S, Dehbozorgi M, Fattahi F, Movahed F, Esfandiari N Heliyon. 2025; 11(3):e42258.

PMID: 40007782 PMC: 11850152. DOI: 10.1016/j.heliyon.2025.e42258.


Study on the Optimization of an Extraction Process of Two Triterpenoid Saponins in the Root of Michx. and Their Protective Effect on Acute Lung Injury.

Mo J, Deng Q, Huang Y, Jia X, Xie F, Zhou B Pharmaceuticals (Basel). 2025; 18(2).

PMID: 40006066 PMC: 11859398. DOI: 10.3390/ph18020253.


Structure characterization of Grifola frondosa polysaccharide and its effect on insulin resistance in HFD-fed mice.

Ding Y, Lan J, Wang Y, Pan Y, Song T, Liu S NPJ Sci Food. 2025; 9(1):3.

PMID: 39774946 PMC: 11707143. DOI: 10.1038/s41538-024-00359-7.


Exploring the Effect and Mechanism of DaYuan Yin Against Acute Lung Injury by Network Pharmacology, Molecular Docking, and Experimental Validation.

Zhang L, Zhu W, Zhang C Drug Des Devel Ther. 2024; 18:5541-5561.

PMID: 39650849 PMC: 11625185. DOI: 10.2147/DDDT.S491521.


Naked Gene Delivery Induces Autophagy for Effective Treatment of Acute Lung Injury in a Mouse Model.

Qin Y, Yu H, Huang Y, Yang X, Li S, Shen A Int J Nanomedicine. 2024; 19:10801-10818.

PMID: 39469449 PMC: 11514649. DOI: 10.2147/IJN.S477947.


References
1.
ASHBAUGH D, Bigelow D, Petty T, LeVine B . Acute respiratory distress in adults. Lancet. 1967; 2(7511):319-23. DOI: 10.1016/s0140-6736(67)90168-7. View

2.
Rubenfeld G, Caldwell E, Peabody E, Weaver J, Martin D, Neff M . Incidence and outcomes of acute lung injury. N Engl J Med. 2005; 353(16):1685-93. DOI: 10.1056/NEJMoa050333. View

3.
Peter J, John P, Graham P, Moran J, George I, Bersten A . Corticosteroids in the prevention and treatment of acute respiratory distress syndrome (ARDS) in adults: meta-analysis. BMJ. 2008; 336(7651):1006-9. PMC: 2364864. DOI: 10.1136/bmj.39537.939039.BE. View

4.
Iwata K, Doi A, Ohji G, Oka H, Oba Y, Takimoto K . Effect of neutrophil elastase inhibitor (sivelestat sodium) in the treatment of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS): a systematic review and meta-analysis. Intern Med. 2010; 49(22):2423-32. DOI: 10.2169/internalmedicine.49.4010. View

5.
Paine 3rd R, Standiford T, Dechert R, Moss M, Martin G, Rosenberg A . A randomized trial of recombinant human granulocyte-macrophage colony stimulating factor for patients with acute lung injury. Crit Care Med. 2011; 40(1):90-7. PMC: 3242850. DOI: 10.1097/CCM.0b013e31822d7bf0. View