Unbiased Proteomic Profiling of Host Cell Extracellular Vesicle Composition and Dynamics Upon HIV-1 Infection

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2021 Mar 12

PMID 33709510

Citations 28

Authors

Lorena Martin-Jaular

Nathalie Nevo

Julia P Schessner

Mercedes Tkach

Mabel Jouve

Florent Dingli

Damarys Loew

Kenneth W Witwer

Matias Ostrowski

Georg H H Borner

Clotilde Thery

Affiliations

Soon will be listed here.

Abstract

Cells release diverse types of extracellular vesicles (EVs), which transfer complex signals to surrounding cells. Specific markers to distinguish different EVs (e.g. exosomes, ectosomes, enveloped viruses like HIV) are still lacking. We have developed a proteomic profiling approach for characterizing EV subtype composition and applied it to human Jurkat T cells. We generated an interactive database to define groups of proteins with similar profiles, suggesting release in similar EVs. Biochemical validation confirmed the presence of preferred partners of commonly used exosome markers in EVs: CD81/ADAM10/ITGB1, and CD63/syntenin. We then compared EVs from control and HIV-1-infected cells. HIV infection altered EV profiles of several cellular proteins, including MOV10 and SPN, which became incorporated into HIV virions, and SERINC3, which was re-routed to non-viral EVs in a Nef-dependent manner. Furthermore, we found that SERINC3 controls the surface composition of EVs. Our workflow provides an unbiased approach for identifying candidate markers and potential regulators of EV subtypes. It can be widely applied to in vitro experimental systems for investigating physiological or pathological modifications of EV release.

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